MORPHOLOGICAL, ANATOMICAL, BIOCHEMICAL, AND DNA BARCODE CHARACTERISTICS OF THE TWO RICE BEAN CULTIVARS COLLECTED IN YEN BAI AND HA GIANG

Abstract

Vigna umbellata, also known as rice bean, is known to provide nutrition to humans and  animals, and is a good green manure for hills. Trees, young leaves and young fruits are used as vegetable seeds. Rice beans collected in the province of Yen Bai (NN01_YB) and Ha Giang (NN02_HG) in the form of creeping, climbing, with many fluffy hair on the bodies. Leaves have three leaflets, heart-shaped with fluffy hair. Flowers are yellow and bloom in the armpits. Internal transcribed spacer (ITS) region isolated from two rice beans in Yen Bai and Ha Giang, Vietnam are 480 bp in length; The genetic similarities of the two cultivars were 99.8% and there were differences in the 3 nucleotide positions. The total protein of the two cultivars NN01_YB and NN02_HG were 30.3 and 30.0 mg/100 grs seeds respectively. The α-amylase activity of the two cultivars NN01_YB and NN02_HG was 1.2 and 1.0 U/mg respectively. The protease activity of two varieties of NN01_YB and NN02_HG was 2.2 and 2.1 U/mg respectively.