CLONE AND SEQUENCE COMPLETE E2 GENE OF CLASSICAL SWINE FEVER VIRUS FROM SEVERAL FARMS IN THE SOUTH VIETNAM

Nguyễn Minh Nam; Nguyễn Thị Phương Bình; Lại Công Danh; Nguyễn Ngọc Hải

doi:10.34238/tnu-jst.6139

CLONE AND SEQUENCE COMPLETE E2 GENE OF CLASSICAL SWINE FEVER VIRUS FROM SEVERAL FARMS IN THE SOUTH VIETNAM

About this article

Received: 09/06/22 Revised: 29/07/22 Published: 31/07/22

Authors

1. Nguyen Minh Nam , Vietnam National University Ho Chi Minh City
2. Nguyen Thi Phuong Binh, P&Y Livestock Service Company Limited, Ho Chi Minh City
3. Lai Cong Danh, Nong Lam University, Ho Chi Minh City
4. Nguyen Ngoc Hai, Nong Lam University, Ho Chi Minh City

Abstract

Classical swine fever is an old and dangerous disease that causes significant economic losses to the swine industry worldwide. E2 protein-coding gene sequencing is an essential basis for assessing and comparing the genetic diversity of CSFV and developing a vaccine against CSFV. This study was conducted to create E. coli cells carrying plasmids containing the full-length E2 gene sequences. Twenty-one CSFV-positive samples were collected that were transformed into E. coli DH5α cells. The plasmids were then purified and sequenced to evaluate the effectiveness of the cloning method. The results showed that the research has successfully created 21 strains of E. coli bacteria carrying the complete E2 gene. These sequences were highly similar (87.94 – 98.84%) compared with the 100 sequences having the highest similarity on NCBI. Besides, 19 sequences had very high similarity with strains belonging to subgroup 2.1c (95.00 - 96.34%) and two sequences with subgroup 2.2 (92.05 - 93.03%). This study is the basis for applying the E. coli cloning technique carrying the E2 gene in stable genetic analysis of CSFV, as well as the basis for the subsequent generation of recombinant E2 proteins for developing an E2-subunit vaccine against CSFV.

Keywords

CSFV; Gene E2; Sequencing; Cloning; Classical swine fever

Full Text:

PDF (Tiếng Việt)

References

[1] P. Lowings, G. Ibata, J. Needham, and D. Paton, "Classical swine fever virus diversity and evolution," Journal of General Virology, vol. 77, no. 6, pp. 1311-1321, 1996.

[2] D. Paton, A. McGoldrick, I. Greiser-Wilke, S. Parchariyanon, J.-Y. Song, P. Liou, T. Stadejek, J. Lowings, H. Björklund, and S. Belak, "Genetic typing of classical swine fever virus," Veterinary microbiology, vol. 73, no. 2-3, pp. 137-157, 2000.

[3] M.-C. Deng, C.-C. Huang, T.-S. Huang, C.-Y. Chang, Y.-J. Lin, M.-S. Chien, and M.-H. Jong, "Phylogenetic analysis of classical swine fever virus isolated from Taiwan," Veterinary microbiology, vol. 106, no. 3-4, pp. 187-193, 2005.

[4] C. Pan, M. Jong, T. Huang, H. Liu, S. Lin, and S. Lai, "Phylogenetic analysis of classical swine fever virus in Taiwan," Archives of Virology, vol. 150, no. 6, pp. 1101-1119, 2005.

[5] W. Gong, J. Wu, Z. Lu, L. Zhang, S. Qin, F. Chen, Z. Peng, Q. Wang, L. Ma, and A. Bai, "Genetic diversity of subgenotype 2.1 isolates of classical swine fever virus," Infection, Genetics and Evolution, vol. 41, pp. 218-226, 2016.

[6] A. Kamakawa, H. T. V. Thu, and S. Yamada, "Epidemiological survey of viral diseases of pigs in the Mekong delta of Vietnam between 1999 and 2003," Veterinary Microbiology, vol. 118, no. 1-2, pp. 47-56, 2006.

[7] A. Töpfer, D. Höper, S. Blome, M. Beer, N. Beerenwinkel, N. Ruggli, and I. Leifer, "Sequencing approach to analyze the role of quasispecies for classical swine fever," Virology, vol. 438, no. 1, pp. 14-19, 2013.

[8] U. Fahnøe, A. G. Pedersen, P. C. Risager, J. Nielsen, G. J. Belsham, D. Höper, M. Beer, and T. B. Rasmussen, "Rescue of the highly virulent classical swine fever virus strain “Koslov” from cloned cDNA and first insights into genome variations relevant for virulence," Virology, vol. 468, pp. 379-387, 2014.

[9] A. Postel, S. Schmeiser, J. Bernau, A. Meindl-Boehmer, G. Pridotkas, Z. Dirbakova, M. Mojzis, and P. Becher, "Improved strategy for phylogenetic analysis of classical swine fever virus based on full-length E2 encoding sequences," Veterinary research, vol. 43, no. 1, pp. 1-15, 2012.

[10] M. Beer, K.V. Goller, C. Staubach, and S. Blome, "Genetic variability and distribution of Classical swine fever virus," Animal Health Research Reviews, vol. 16, no. 1, pp. 33-39, 2015.

[11] M. König, T. Lengsfeld, T. Pauly, R. Stark, and H.-J. Thiel, "Classical swine fever virus: independent induction of protective immunity by two structural glycoproteins," Journal of virology, vol. 69, no. 10, pp. 6479-6486, 1995.

[12] A. Clavijo, M. Lin, J. Riva, and E.-M. Zhou, "Application of competitive enzyme-linked immunosorbent assay for the serologic diagnosis of classical swine fever virus infection," Journal of veterinary diagnostic investigation, vol. 13, no. 4, pp. 357-360, 2001.

[13] H. Zhang, W. Wen, Z. Zhao, J. Wang, H. Chen, P. Qian, and X. Li, "Enhanced protective immunity to CSFV E2 subunit vaccine by using IFN-γ as immunoadjuvant in weaning piglets," Vaccine, vol. 36, no. 48, pp. 7353-7360, 2018.

[14] S. Yao, D. J. Hart, and Y. An, "Recent advances in universal TA cloning methods for use in function studies," Protein Engineering, Design and Selection, vol. 29, no. 11, pp. 551-556, 2016.

[15] Y. Qi, B.-Q. Zhang, Z. Shen, and Y.-H. Chen, "Candidate vaccine focused on a classical swine fever virus epitope induced antibodies with neutralizing activity," Viral immunology, vol. 22, no. 3, pp. 205-213, 2009.

[16] X.-N. Dong and Y.-H. Chen, "Marker vaccine strategies and candidate CSFV marker vaccines," Vaccine, vol. 25, no. 2, pp. 205-230, 2007.

[17] A. Bouma, A. De Smit, E. De Kluijver, C. Terpstra, and R. Moormann, "Efficacy and stability of a subunit vaccine based on glycoprotein E2 of classical swine fever virus," Veterinary microbiology, vol. 66, no. 2, pp. 101-114, 1999.

[18] M. Hulst, D. Westra, G. Wensvoort, and R. Moormann, "Glycoprotein E1 of hog cholera virus expressed in insect cells protects swine from hog cholera," Journal of Virology, vol. 67, no. 9, pp. 5435-5442, 1993.

[19] P. Van Rijn, A. Bossers, G. Wensvoort, and R. Moormann, "Classical swine fever virus (CSFV) envelope glycoprotein E2 containing one structural antigenic unit protects pigs from lethal CSFV challenge," Journal of General Virology, vol. 77, no. 11, pp. 2737-2745, 1996.

[20] G. Wensvoort, "Topographical and functional mapping of epitopes on hog cholera virus with monoclonal antibodies," Journal of general virology, vol. 70, no. 11, pp. 2865-2876, 1989.

[21] N. H. Nguyen, B. T. P. Nguyen, D. T. Do, T. Q. Nguyen, D. T. M. Nguyen, and M. N. Nguyen, "Genetic diversity and molecular characterization of classical swine fever virus envelope protein genes E2 and Erns circulating in Vietnam from 2017 to 2019," Infection, Genetics and Evolution, vol. 96, no. p. 105140, 2021.

[22] T. T. P. Le, T. T. H. Nguyen, V. P. Kim, X. H. Tran, A. T. Bui, H. Dang, D. T. Tran, C. J. Morrissy, F. Wong, V. Stevens, and K. Davies, "Genetic analysis of swine cholera virus in some southern provinces of Vietnam," Journal of Veterinary science and technology, vol. XVII, no. 2, pp. 5-11, 2010.

DOI: https://doi.org/10.34238/tnu-jst.6139

Refbacks

There are currently no refbacks.



Remember me