RESEARCH ON USING IN VITRO TECHNIQUES TO PROPAGATE LADY SLIPPER ORCHID (PAPHIOPEDILUM TRANLIENIANUM)

Nguyễn Thị Tình; Nguyễn Tiến Dũng; Bùi Tri Thức; Dương Thị Thu Hoài; Lưu Hồng Sơn; Lê Thanh Ninh; Trần Ngọc Hùng; Ngô Xuân Bình

doi:10.34238/tnu-jst.6367

RESEARCH ON USING IN VITRO TECHNIQUES TO PROPAGATE LADY SLIPPER ORCHID (PAPHIOPEDILUM TRANLIENIANUM)

About this article

Received: 16/08/22 Revised: 15/09/22 Published: 15/09/22

Authors

1. Nguyen Thi Tinh, Vietnam Academy of Agricultural Sciences; TNU - University of Agriculture and Forestry
2. Nguyen Tien Dung, TNU - University of Agriculture and Forestry
3. Bui Tri Thuc, TNU - University of Agriculture and Forestry
4. Duong Thi Thu Hoai, TNU - University of Agriculture and Forestry
5. Luu Hong Son , TNU - University of Agriculture and Forestry
6. Le Thanh Ninh, TNU - University of Agriculture and Forestry
7. Tran Ngoc Hung, Fruit and Vegetable Research Institute
8. Ngo Xuan Binh, TNU - University of Agriculture and Forestry

Abstract

The difference between lady's slipper orchids and others is easy to realize beause of the special morphology of flowers. There is only one sac-like petal, as a slipper in the flower, therefore this orchids species named as "lady's slipper orchids". Paphiopedilum tranlienianum is one of endemic orichids in Vietnam. The fruits, which were pollinated after 9 months, were selected for experiments. The embryos were cultivated and germinated on MS medium, supplemented with 30 g/l sugar, 1 g/l activated carbon, 5.5 g/l agar, 150 ml/l coconut water. The rapid multiplication phase was supplemented with growth regulator as: BAP 4 mg/l or BAP 4 mg/l, combined with 0.4-0.6 mg/l kinetin. In this media, the hormone had a great influence on the ability to create many buds with the coefficient of germination is 79; Shoot height is 2.46 cm; tree is fat and green. The root is induced the most in the MS medium which was supplemented with 30 g/l sugar, 5.5 g/l agar, 150 ml/l coconut water and 1 mg/l concentration of NAA or IAA, which can enhance the growth of root in vitro.

Keywords

Slipper Orchird - Paphiopedilum malipoense; In vitro culture; Bud regeneration; NAA; PLBs

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DOI: https://doi.org/10.34238/tnu-jst.6367

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