COMPARISION THE ANTIFUNGAL ACTIVITY AGAINST FUSARIUM OXYSPORUM OF 12 DIFFERENT ESSENTIAL OIL ENCAPSULATED  IN NANOEMULSION AND THEIR COMBINATION

Nguyễn Minh Hiệp; Trần Thị Ngọc Mai

doi:10.34238/tnu-jst.6871

COMPARISION THE ANTIFUNGAL ACTIVITY AGAINST FUSARIUM OXYSPORUM OF 12 DIFFERENT ESSENTIAL OIL ENCAPSULATED IN NANOEMULSION AND THEIR COMBINATION

About this article

Received: 04/11/22 Revised: 20/12/22 Published: 27/12/22

Authors

1. Nguyen Minh Hiep , Center of Radiation Technology and Biotechnology - Nuclear Research Institute
2. Tran Thi Ngoc Mai, Center of Radiation Technology and Biotechnology - Nuclear Research Institute

Abstract

The study aims to evaluate the in vitro antifungal activity against Fusarium oxysporum of 12 plant essential oils encapsulated nanoemulsion (EO-LNs) and their binary and ternary combined formulations of the 4 EOs having the highest in vitro antifungal activity. Firstly, 12 EO-LNs were prepared by a combining method of homogenization and sonication. Their binary and ternary combined formulations were also prepared by mixing with an equal volume ratio of each EO-LNs. In vitro antifungal activity of 12 EO-LNs and their combined formulations was evaluated using petri dish assay. The results indicated that 12 EO-LNs and their combined formulations were successfully prepared with the mean particle size at the nanoscale and the zeta potential greater than -42 mV. The results also showed that the combined formulations of EO-LNs had better antifungal activity against F. oxysporum compared to the corresponding individual EO-LNs. Specially, naTS exhibited the highest in vitro antifungal effect with the growth inhibition value of 88.14% at 500 times dilution. Therefore, naTS has a great potential application as an effective biofungicide against F. oxysporum.

Keywords

Essential oil; Nanoemulsion; Fusarium oxysporum; Antifungal; Combination

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DOI: https://doi.org/10.34238/tnu-jst.6871

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