SPERM CRYOPRESERVATION OF IMPORTED COMMON CARP  (Cyprinus carpio) IN LIQUID NITROGEN

Kim Thị Thoa; Trần Thị Mai Hương; Phạm Hồng Nhật; Lê Văn Khôi; Mai Văn Tài

doi:10.34238/tnu-jst.7095

SPERM CRYOPRESERVATION OF IMPORTED COMMON CARP (Cyprinus carpio) IN LIQUID NITROGEN

About this article

Received: 14/12/22 Revised: 16/05/23 Published: 16/05/23

Authors

1. Kim Thi Thoa , Research Institute for Aquaculture No.1 (RIA1)
2. Tran Thi Mai Huong, Research Institute for Aquaculture No.1 (RIA1)
3. Pham Hong Nhat, Research Institute for Aquaculture No.1 (RIA1)
4. Le Van Khoi, Research Institute for Aquaculture No.1 (RIA1)
5. Mai Van Tai, Research Institute for Aquaculture No.1 (RIA1)

Abstract

Common carp is one of the most important cultured freshwater fish in the world and Vietnam. The objective of this study was to determine the optimal conditions of extender, dilution ratio and freezing method in sperm cryopreservation of two imported common carp (Cyprinus carpio) strains (Szarvas P3 and Tata) from Hungary. Sperm were diluted in different extenders (Kurokura 2 solution and grayling solution with glucose) at dilution ratios of 1:3 and 1:8 (semen: extender) and were frozen using two freezing methods: controlled-rate programmable freezer or above liquid nitrogen vapour. The results showed that using Kurokura 2 extender with dilution ratio of 1:8 (semen: extender), 10% methanol and programmable freezer (from 4°C to -4°C: 4°C min-'; -4°C to -80°C: 11°C min-', and from -80°C they were plunged directly into liquid nitrogen) after stored for 1 month has the highest post-thaw motility (p<0.05) of two strains at 80.8% (Szarvas P3 strain) and 78.3% (Tata strain) compared with fresh sperm 85.8% và 85.4%% respectively.

Keywords

Cryopreservation; Common carp; Cyprinus carpio; Extender; Freezing method

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References

[1] N. K. Agarwal, “Cryopreservation of Fish Semen,” Himal. Aquat. Biodivers. Conserv. New Tools Biotechnol., 2011, pp. 104-127.

[2] T. R. Tiersch, H. Yang, J. A. Jenkins, and Q. Dong, “Sperm cryopreservation in fish and shellfish,” Spermatology, vol. 65, pp. 493-508, 2007.

[3] I. Magyary, B. Urbányi, and L. Horváth, “Cryopreservation of common carp (Cyprinus carpio L.) sperm II. Optimal conditions for fertilization,” Journal of Applied Ichthyology, vol. 12, no. 2. pp. 117-119, 1996.

[4] S. Martínez-Páramo et al., “Cryobanking of aquatic species HHS Public Access,” Aquaculture, vol. 472. pp. 156-177, 2017.

[5] T. T. Kim, T. M. Tran, Q. H. Le, H. N. Pham, V. D. Nguyen, D. N. Ngo and V. M. Nguyen, “Applied Nito-method techonology for cryobanking of aquatic species in Vietnam (in Vietnamese). Final report, Research Institute for Aquaculture No.1, 2010.

[6] T. T. Kim, H. N. Pham, H. D. Nguyen, T. B. N. Tran, N. S. Hoang, V. D. Nguyen, V. T. Khuong, Q. T. Trinh, A. T. Le, “Cryopressevation of some important aquatic species in Vietnam (in Vietnamese), Final report, Research Institute for Aquaculture No.1, 2014.

[7] H. N. Pham, T. T. Kim, V. D. Nguyen, T. T. T. Nguyen and H. D. Nguyen, “Sperm cryopressevation of aquatic animals in Vietnam (in Vietnamese), Science technology in Aquaculture, 2016, pp. 1–10.

[8] Á. Horváth, E. Miskolczi, and B. Urbányi, “Cryopreservation of common carp sperm,” Aquatic Living Resources, vol. 16, no. 5. pp. 457–460, 2003.

[9] Y. Bozkurt, İ. Yavas, and F. Karaca, “Cryopreservation of Brown Trout (Salmo trutta macrostigma) and Ornamental Koi Carp (Cyprinus carpio) Sperm,” in Current Frontiers in Cryopreservation, 2012, pp. 293-304.

[10] M. M. Hassan, M. Nahiduzzaman, S. N. Al Mamun, M. A. Taher, and M. A. R. Hossain, “Fertilization by refrigerator stored sperm of the Indian major carp, Labeo calbasu (Hamilton, 1822),” Aquac. Res., vol. 45, pp. 150-158, 2014.

[11] E. Akçay, Y. Bozkurt, S. Seçer, and N. Tekin, “Cryopreservation of Mirror Carp Semen,” Turkish J. Vet. Anim. Sci., vol. 28, pp. 837-843, 2004.

[12] H. Kurokura, R. Hirano, M. Tomita, and M. Iwahashf, “Cryopreservation of Carp Sperm,” Aquaculture, vol. 37, pp. 267-273, 1984.

[13] E. Lubzens et al., “Carp (Cyprinus carpio L.) spermatozoa cryobanks - Strategies in research and application,” Aquaculture, vol. 155, no. 1-4, pp. 13-30, 1997.

[14] S. Bibi et al., “Identification of suitable extender and cryoprotectant for cryopreservation of Labeo rohita milt,” Aquac. Res., vol. 53, no. 17, pp. 5857-5864, 2022.

[15] M. Sultana, M. Nahiduzzaman, M. M. Hassan, M. U. H. Khanam, and M. A. R. Hossain, “Fertility of cryopreserved common carp (Cyprinus carpio) spermatozoa,” Rajshahi Univ. Zool. Soc., vol. 28, pp. 51-55, 2010.

[16] F. Lahnsteiner, B. Berger, A. Horvath, B. Urbanyi, and T. Weismann, “Cryopreservation of Spermatozoa Ln Cyprinid Fishes,” Theriogenology, vol. 54, pp. 1477-1498, 2000.

[17] G. Bernáth et al., “Improvement of Common Carp (Cyprinus Carpio) Sperm Cryopreservation Using a Programable Freezer,” Gen. Comp. Endocrinol., vol. 237, pp. 78-88, 2016.

[18] S. S. du Plessis, A. Agarwal, G. Mohanty, and M. van der Linde, “Oxidative phosphorylation versus glycolysis: what fuel do spermatozoa use?,” Asian J. Androl., vol. 17, pp. 230-235, 2015.

[19] R. I. Arifiantini, T. L. Y. B. Purwantara, and D. Sajuthi, “Effect of Different Cryoprotective Agents on Skim Milk and Dimitropoulus Extender for Stallion Semen Cryopreservation,” J. Indones. Trop. Anim. Agric., vol. 35, pp. 68-74, 2010.

[20] V. T. Do, “Research on sperm characters and cypropreservation of goat in Vietnam (in Vietnamese),” PhD. Thesis, Institute of Biotechnology, Hanoi, 153 p., 2000.

[21] H. Irawan, V. Vuthiphandchai, and S. Nimrat, “The effect of extenders, cryoprotectants and cryopreservation methods on common carp (Cyprinus carpio) sperm,” Animal Reproduction Science, vol. 122, no. 3-4, pp. 236-243, 2010.

DOI: https://doi.org/10.34238/tnu-jst.7095

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