STANDARDIZING THE CULTURE ENVIRONMENT AND TECHNIQUE FORMOUSE EMBRYONIC STEM CELLS APPLIED IN THE LABORATORY AT THAI NGUYEN UNIVERSITY OF MEDICINE AND PHARMACY

Abstract

Stem cell research in the field of medicine has been started from successful embryonic stem cell culture. However, depending on the actual conditions of each laboratory, researchers have built their own stem cell culture environment and culture technique.

In this research, we have studied the conditions and techniques of stem cell culture from domestic and foreign laboratories. Then we standardize the environment and techniques of mouse embryonic stem cell culture is applied in the laboratory in TNU - University of Medicine and Pharmacy.

We have successfully standardized the environment and techniques of mouse embryonic stem cell culture. Successful transplantation of mouse embryonic stem cell line. Well-formed, morphologically-normal cells, which are capable of targeting differentiated cells into desired cells.