BIOLOGICAL CHARACTERISTICS OF SPHINGOBIUM LIMNETICUM BPTC-107 SHOWING ABILITY OF PESTICIDE BIODEGRADATION

Nguyễn Mạnh Tuấn; Đỗ Thị Hiền; Đỗ Bích Duệ; Hoàng Văn Hưng

doi:10.34238/tnu-jst.9045

BIOLOGICAL CHARACTERISTICS OF SPHINGOBIUM LIMNETICUM BPTC-107 SHOWING ABILITY OF PESTICIDE BIODEGRADATION

About this article

Received: 24/10/23 Revised: 05/01/24 Published: 05/01/24

Authors

1. Nguyen Manh Tuan , TNU - Institute of Life Science
2. Do Thi Hien, TNU - Institute of Life Science
3. Do Bich Due, TNU - Institute of Life Science
4. Hoang Van Hung, TNU - Institute of Life Science

Abstract

The aim of this study is to molecularly identify and determine cultural characteristics, as well as ability to grow in media supplemented with the pesticides of strain BPTC-107. Results of 16S rRNA gene sequence comparison and analysis showed that strain BPTC-107 was Sphingobium limneticum. Strain BPTC-107 was capable of growing in Tryptone Soya Broth, R2A and Nutrient Broth media (Tryptone Soya Broth medium was optimal), growing in the temperature range of 15-40^oC (25-30^oC was optimal). Strain BPTC-107 was able to grow at pH 4-8 (pH 7 was optimal). Strain BPTC-107 was capable of using sugar sources including D-saccharose, D-maltose, L-rhamnose, D-manitol, D-glucose and 3-hydroxybenzoic acid for growth. Alkaline phosphatase, valine arylamidase, leucine arylamidase, α-galactosidase and lipase (C14) were enzymes produced by strain BPTC-107. Furthermore, strain BPTC-107 was capable of growing in Mineral Salts Medium (MSM) supplemented with pesticides at concentrations of 10, 50 and 100 with OD values of 0.08 ± 0.03; 0.15 ± 0.03 and 0.10 ± 0.02, respectively (of which 50 mg/l was optimal concentration for growth).

Keywords

Identification; Biodegradation of pesticides; Isolation; Sphingobium; Pesticide residues

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DOI: https://doi.org/10.34238/tnu-jst.9045

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