RESEARCH ON PROCESS FOR PREPARATION OF ENTVN HARD CAPSULES CONTAINING PROBIOTIC ENTEROCOCCUS FAECIUM

Bùi Thị Lan Anh; Hoàng Giang; Đào Nguyên Mạnh

doi:10.34238/tnu-jst.9051

RESEARCH ON PROCESS FOR PREPARATION OF ENTVN HARD CAPSULES CONTAINING PROBIOTIC ENTEROCOCCUS FAECIUM

About this article

Received: 25/10/23 Revised: 25/03/24 Published: 29/03/24

Authors

1. Bui Thi Lan Anh , Vietnam – Russia Tropical Center
2. Hoang Giang, Hanoi University of Science - VNU
3. Dao Nguyen Manh, Vietnam – Russia Tropical Center

Abstract

The study presents the process of preparing EntVN hard capsules from Enterococcus faecium F26BA and F54BA probiotic strains isolated from Vietnamese food sources. The concentration of pasteurized milk to create starter biomass is 15% (w/w), the time to freeze dry the wet biomass is 24 hours, the ratio of filler excipient (soybean powder) to probiotic biomass is 1:4 (biomass: excipients, w/w), probiotic biomass powder is obtained with colony density of 4.5x10¹⁰CFU/g after 4 hours at 40°C drying temperature. Biomass powder (460 mg/capsule) after fine grinding mixed with filler excipients (20 mg/capsule), adhesive excipients (10 mg/capsule), smoothing excipients (10 mg/capsule), then encapsulation is proceeded. The obtained EntVN capsules met the criteria of weight (error no more than 7.5%), disintegration (less than 30 minutes), infection limit, heavy metal content and number of colonies (3.3x10¹⁰CFU/g). EntVN capsules are prepared from indigenous probiotic strains with characteristics that are easily adapted and suitable for Vietnamese people, as well as common ingredients, simple process and moderate price for local market.

Keywords

Enterococcus faecium F26BA; Enterococcus faecium F54BA; Hard capsule; Probiotic; EntVN 500 mg

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References

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DOI: https://doi.org/10.34238/tnu-jst.9051

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