ISOLATION OF BACTERIA FROM SHRIMP POND WASTEWATER WITH THE CAPACITY TO INHIBIT Pseudomonas aeruginosa ATCC 27853   AND Vibrio parahaemolyticus ATCC 10885

Trương Thị Bích Vân; Trần Thị Liễu

doi:10.34238/tnu-jst.9149

ISOLATION OF BACTERIA FROM SHRIMP POND WASTEWATER WITH THE CAPACITY TO INHIBIT Pseudomonas aeruginosa ATCC 27853 AND Vibrio parahaemolyticus ATCC 10885

About this article

Received: 04/11/23 Revised: 25/03/24 Published: 03/04/24

Authors

1. Truong Thi Bich Van , Institute of Food and Biotechnology - Can Tho University
2. Tran Thi Lieu, Institute of Food and Biotechnology - Can Tho University

Abstract

Six bacteriophage strains (Bacteriophage; TKT) ФPVtH, ФPCK1, ФPVCt3, ФPVCt4, ФPDT4, ФPDT3 that can lyse Pseudomonas aeruginosa ATCC 27853 were determined by the double-layer agar method. The results of the host spectrum survey with five bacterial species Vibrio parahaemolyticus NCTC 10884, Vibrio parahaemolyticus NCTC 10885, Vibrio furnissii NCTC 11218, Vibrio parahaemolyticus ATCC 17802, Vibrio vulnificus ATCC 29307, showed that all six phage strains produced stains soluble on Vibrio parahaemolyticus NCTC 10885. The results of all six bacteriophage strains have affected the growth of Pseudomonas aeruginosa ATCC 27853 through the OD600 measurement method and the bacterial population spread method. Six bacteriophage strains reduce the host bacterial population by up to 90%, of which ФPCK1 reduces the highest bacterial population by 98%. The study results show that the use of bacteriophages has the potential to control pathogens and harmful bacteria such as Pseudomonas spp. and Vibrio spp.

Keywords

Double-layer agar; Pseudomonas spp.; Bacterial host; Vibrio spp.; Bacteriophage

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DOI: https://doi.org/10.34238/tnu-jst.9149

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