SURVEY ON ANTIBIOTIC RESISTANCEOF Salmonella spp. ISOLATED FROM FERMENTED PORK ROLLS VINH LONG PROVINCE | Yến | TNU Journal of Science and Technology

SURVEY ON ANTIBIOTIC RESISTANCEOF Salmonella spp. ISOLATED FROM FERMENTED PORK ROLLS VINH LONG PROVINCE

About this article

Received: 13/11/23                Revised: 05/01/24                Published: 03/02/24

Authors

1. Dang Phi Yen Email to author, Vinh Long University of Technology Education
2. Quach Van Cao Thi, Vinh Long University of Technology Education
3. Nguyen Trung Truc, Vinh Long University of Technology Education

Abstract


Antibiotic resistance of bacteria in food is one of the major safety concerns for human health, including antibiotic resistance of Salmonella spp. The study was conducted to investigate the antibiotic resistance of Salmonella spp. bacteria in fermented pork rolls at 10 traditional markets in Vinh Long province. The study isolated 17 bacterial strains, and16S rRNA gene sequencing results showed that two bacterial strains Sal7, and Sal15 were 100% similar to Salmonella enterica strain LHST_2018, andSalmonella enterica strain SEWLSH-13 respectively. These results showed that the bacteria in the study were sensitive to the antibiotics norfloxacin, ampicillin/sulbactam, and ceftazidime at rates of 100%, 94.12%, and 94.12% respectively. The bacterial strains isolated in the study all showed resistance to tested antibiotics such as ampicillin, gentamicin, doxycycline, ciprofloxacin, trimethoprim/sulfamethoxazole, and nalidixic acid. In there, ampicillin is the antibiotic with the highest antibiotic resistance rate, accounting for 88.24%.Among the 100% of the isolates that were resistant to at least one antibiotic, the multidrug resistance MAR index ranges from 0.21 to 0.64.

Keywords


Antibiotic resistance; Fermented pork rolls; Multidrug resistance; Salmonella spp.; Vinh Long

References


[1] T. H. N. Duong, T. H. Mai, B. H. Lu, T. X. M. Ly and T. Q. H. Bui, "Isolation and selection of lactic acid bacteria strains Nem Chua from meat with potential application as seed bacteria in Nem Chua production," (in Vietnamese), Can Tho University Science Journal, vol. 59, no. 3, pp. 86-93, 2023.

[2] T. M. Le, T. N. Do, M. H. Le, T. M. K. Chan, and V. Q. Van, "Survey on safety and hygiene of Nem chua in Hanoi," (in Vietnamese), Vietnam Journal of Science Technology, vol. 50, no. 2, pp. 231-231, 2012.

[3] M. Rega, I. Carmosino, P. Bonilauri, V. Frascolla, A. Vismarra, and C. Bacci, "Prevalence of ESβL, AmpC and Colistin-Resistant E. coli in Meat: A Comparison between Pork and Wild Boar," Microorganisms, vol. 9, no. 2, pp. 1-11, 2021.

[4] T. N. Nguyen, T. B. V. Nguyen, V. C. Nguyen et al., "Antimicrobial residues and resistance against critically important antimicrobials in non-typhoidal Salmonella from meat sold at wet markets and supermarkets in Vietnam," (in Vietnamese), International Journal of Food Microbiology, vol. 266, pp. 301-309, 2018.

[5] S. Ali and A. F. Alsayeqh, "Review of major meat-borne zoonotic bacterial pathogens," Frontiers in Public Health, vol. 10, 2022, Art. no. 1045599.

[6] Y. Tian, D. Gu, F. Wang et al., "Prevalence and Characteristics of Salmonella spp. from a Pig Farm in Shanghai, China," Foodborne pathogens and disease, vol. 18, no. 7, pp. 477-488, 2021.

[7] H. Song and A. Zhu, "Isolation and Identification of Salmonella in Pork," Methods in molecular biology, vol. 2182, pp. 197-203, 2021.

[8] X. H. Nguyen, "Assessing the level of bacterial contamination in pork from animal import-export companies and slaughterhouses for domestic consumption," (in Vietnamese), Hue University Journal of Science: Agriculture and Rural Development, vol. 108, no. 9, 2015. [Abstract]. Available: ProQuest, https://jos.hueuni.edu.vn/. [Accessed November 1, 2023].

[9] H. A. V. Truong, V. H. Chu, Y. H. Huynh, and H. K. T. Nguyen, "Antimicrobial susceptibility of Salmonella spp. isolated from raw meats at traditional markets in Ho Chi Minh city," (in Vietnamese), Journal of Science and Technology Viet Nam, vol. 63, no. 8, pp. 55-59, 2021.

[10] EFSA, "The European Union Summary Report on Antimicrobial Resistance in zoonotic and indicator bacteria from humans, animals and food in 2019-2020," EFSA Journal, European Food Safety Authority, vol. 20, no. 3, 2022, Art. no. e07209.

[11] P. Leekitcharoenphon, M. H. K. Johansson, P. Munk et al., "Genomic evolution of antimicrobial resistance in Escherichia coli," Scientific reports, vol. 11, no. 1, 2021, Art. no. 15108.

[12] J. Hur, C. Jawale, and J. H. Lee, "Antimicrobial resistance of Salmonella isolated from food animals: A review," Food Research International, vol. 45, no. 2, pp. 819-830, 2012.

[13] M. Rega, L. Andriani, A. Poeta, S. Bonardi, M. Conter, and C. Bacci, "The Pork Food Chain as a Route of Transmission of Antimicrobial Resistant Escherichia coli: A Farm-to-Fork Perspective," Antibiotics (Basel, Switzerland), vol. 12, no. 2, pp. 1-14, 2023.

[14] J. Campos, J. Mourão, L. Peixe, and P. Antunes, "Non-typhoidal Salmonella in the Pig Production Chain: A Comprehensive Analysis of Its Impact on Human Health," Pathogens (Basel, Switzerland), vol. 8, no. 1, pp. 1-28, 2019.

[15] M. Cheesbrough, Medical laboratory manual for tropical countries, 14 Bevills Close, Doddington, Cambridgeshire, PE15 OTT., 1981.

[16] F. Sharmin and M. Rahman, "Isolation and characterization of protease producing Bacillus strain FS-1," Agricultural Engineering International: The CIGR Ejournal, vol. 9, pp. 1-10, 2007.

[17] A. W. Bauer, W. M. Kirby, J. C. Sherris, and M. Turck, "Antibiotic susceptibility testing by a standardized single disk method," Technical bulletin of the Registry of Medical Technologists. American Society of Clinical Pathologists. Registry of Medical Technologists, vol. 36, no. 3, pp. 49-52, 1966.

[18] Clinical and Laboratory Standards Institute (CLSI), "Performance Standards for Antimicrobial Susceptibility Testing (M100)," Clinical and Laboratory Standards Institute, 2020. [Online]. Available: http://em100.edaptivedocs.net/dashboard.aspx. [Accessed Sept. 22, 2023].

[19] M. H. G. Kanaan and M. T. Abdulwahid, "Prevalence rate, antibiotic resistance and biotyping of thermotolerant Campylobacter isolated from poultry products vended in Wasit markets," Current Research in Nutrition and Food Science Journal, vol. 7, no. 3, pp. 905-917, 2019.

[20] P. H. Krumperman, "Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods," Applied and environmental microbiology, vol. 46, no. 1, pp. 165-70, 1983.

[21] W. G. Weisburg, S. M. Barns, D. A. Pelletier, and D. J. Lane, "16S ribosomal DNA amplification for phylogenetic study," Journal of bacteriology, vol. 173, no. 2, pp. 697-703, 1991.

[22] S. M. Yanestria, R. P. Rahmaniar, F. J. Wibisono, and M. H. Effendi, "Detection of invA gene of Salmonella from milkfish (Chanos chanos) at Sidoarjo wet fish market, Indonesia, using polymerase chain reaction technique," Veterinary world, vol. 12, no. 1, pp. 170-175, 2019.

[23] A. P. Soubeiga, D. S. Kpoda, M. K. A. Compaoré et al., "Molecular Characterization and the Antimicrobial Resistance Profile of Salmonella spp. Isolated from Ready-to-Eat Foods in Ouagadougou, Burkina Faso," International Journal of Microbiology, vol. 2022, 2022, Art. no. 9640828.

[24] L. Guo, T. Xiao, L. Wu et al., "Comprehensive profiling of serotypes, antimicrobial resistance and virulence of Salmonella isolates from food animals in China, 2015-2021," Frontiers in microbiology, vol. 14, 2023, Art. no. 1133241.

[25] T. Eguale, J. Birungi, D. Asrat et al., "Genetic markers associated with resistance to beta-lactam and quinolone antimicrobials in non-typhoidal Salmonella isolates from humans and animals in central Ethiopia," Antimicrobial resistance and infection control, vol. 6, p. 13, 2017.

[26] Q. V. Tran, V. L. Ho, and X. H. Nguyen "Biological characteristics of Salmonella in infected ducks raised in hoai nhon district, binh dinh province," Hue University Journal of Science: Agriculture Rural Development, vol. 126, no. 3A, pp. 99-106, 2017.

[27] V. M. H. Nguyen, T. V. Nguyen, J. I. Campbell, S. Baker, C. T. Nguyen, and T. P. T. Phan, " Prevalence and antibiotic resistance pattern of Salmonella spp. isolated from farms at Dak Lak province," Science Technology, vol. 18, no. T5-2015, pp. 85-94, 2015.

[28] M. S. Ramirez and M. E. Tolmasky, "Aminoglycoside modifying enzymes," Drug Resistance Updates, vol. 13, no. 6, pp. 151-71, 2010.

[29] T. H. T. Nguyen, T. V. Nguyen, T. N. Q. Nguyen, N. M, Nghiem and T. B. T. Vo, "Evaluation of antibiotic resistance genes expression in food poisoning Salmonella typhimurium isolated from retail pork in Ha Noi," Academia Journal of Biology, vol. 39, no. 2, pp. 210-218, 2017.

[30] R. E. Castro-Vargas, M. P. Herrera-Sánchez, R. Rodríguez-Hernández, and I. S. Rondón-Barragán, "Antibiotic resistance in Salmonella spp. isolated from poultry: A global overview," Veterinary world, vol. 13, no. 10, pp. 2070-2084, 2020.

[31] X. Y. Ho, K. T. Nguyen, and T. L. K. Ly, "Survey of Salmonella spp. on chickens and the environment in some farming households in Vinh Long province," (in Vietnamese), Can Tho University Science Journal, vol. 55, no. 6, pp. 1-6, 2019.

[32] S. Guo, M. Y . F Tay, K. T. Aung et al., "Phenotypic and genotypic characterization of antimicrobial resistant Escherichia coli isolated from ready-to-eat food in Singapore using disk diffusion, broth microdilution and whole genome sequencing methods," Food Control, vol. 99, pp. 89-97, 2019.

[33] B. Tang, M. Elbediwi, R. B. Nambiar, H. Yang, J. Lin, and M. Yue, "Genomic Characterization of Antimicrobial-Resistant Salmonella enterica in Duck, Chicken, and Pig Farms and Retail Markets in Eastern China," Microbiology spectrum, vol. 10, no. 5, 2022, Art. no. e0125722.

[34] T. V. Nguyen, N. M. Nghiem, and T. B. T. Vo "Determination of antibiotic resistance of Salmonella isolated from pork, beef, and chicken meat at the retail markets in Hanoi," Vietnam Journal of Biotechnology, vol. 16, no. 3, pp. 553-564, 2018.

[35] P. H. Serrano, Responsible use of antibiotics in aquaculture. Food & Agriculture Org., 2005.

[36] A. Siddique, S. Azim, A. Ali et al., "Antimicrobial Resistance Profiling of Biofilm Forming Non Typhoidal Salmonella enterica Isolates from Poultry and Its Associated Food Products from Pakistan," Antibiotics (Basel, Switzerland), vol. 10, no. 7, pp. 1-14, 2021.




DOI: https://doi.org/10.34238/tnu-jst.9197

Refbacks

  • There are currently no refbacks.
TNU Journal of Science and Technology
Rooms 408, 409 - Administration Building - Thai Nguyen University
Tan Thinh Ward - Thai Nguyen City
Phone: (+84) 208 3840 288 - E-mail: jst@tnu.edu.vn
Based on Open Journal Systems
©2018 All Rights Reserved