EFFECT OF TEA SIZE ON THE ANTIBACTERIAL, ANTIOXIDANT,  AND CANCER CELL TOXIC ACTIVITIES OF GREEN TEA

Vì Thị Xuân Thủy; Đặng Xuân Hoàng; Vũ Đức Toàn; Nguyễn Nam Anh; Đoàn Nguyễn Phú

doi:10.34238/tnu-jst.9363

EFFECT OF TEA SIZE ON THE ANTIBACTERIAL, ANTIOXIDANT, AND CANCER CELL TOXIC ACTIVITIES OF GREEN TEA

About this article

Received: 07/12/23 Revised: 05/03/24 Published: 11/03/24

Authors

1. Vi Thi Xuan Thuy , Tay Bac University
2. Dang Xuan Hoang, Tay Bac University
3. Vu Duc Toan, Tay Bac University
4. Nguyen Nam Anh, Department of Education and Training of Son La province
5. Doan Nguyen Phu, Department of Education and Training of Son La province

Abstract

Tea is the second most commonly consumed beverage in the world. Green tea is the best for cancer prevention; it is an antioxidant, anti-inflammatory, and antibacterial. This study aims to analyse the effect of size on the biological activity of green tea to find a healthy way to drink green tea. Using quality analysis methods and testing for antibacterial, antioxidant and toxic to cancer cell lines, show that green tea samples measuring 2-3 mm dipped in hot water (100^oC) for 5 minutes ensure sensory quality. The antibacterial ability (200 mg/mL) of small ground sample (2-3 mm) in Escherichia coli (ATCC 25922) was 1.6 times higher than that of unground green tea samples, Staphylococcus aureus 2.5 times, Pseudomonas aeruginosa 1.64 times. At a 128 µg/mL concentration, a 2-3mm green tea sample reduced DPPH free radicals at 68%, and an unground green tea sample at 63%. At a residual concentration of 256 µg/mL, the 2-3 mm green tea sample inhibited the growth of cancer cell lines higher than the unground sample, HepG2 (liver cancer) 3.45 times, KB (carcinoma) 3.21 times, A549 cell line (lung cancer) 2.98 times, MCF-7 (breast cancer) 2.43 times.

Keywords

Antioxidant; Antibacterial; Cancer cell toxin; Finely ground; Green tea

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References

[1] Z. Chen and Z. Lin, “Tea and human health: biomedical functions of tea active components and current issues,” Journal of Zhejiang University-Science B (Biomedicine & Biotechnology), vol. 16, no. 2, pp. 87-102, 2015.

[2] International tea market, Market situation, prospectsa and emerging issues, Food and Agriculture Organization of the United Nations, Rome, 2022.

[3] G. B. Sonara, D. P. Shah, T. N. Gheewala, and S. P. Roy, “Biological Effects of Tea Polyphenols on Human Health: A Review,” International Research Journal of Plant Science, vol. 14, no. 1, pp. 01-10, 2023.

[4] M. Aviram, L. Dornfeld, and M. Rosenblat, “Pomegranate juice consumption reduces oxidative stress, atherogenic modifications to LDL, and platelet aggregation: Studies in humans and in atherosclerotic apolipoprotein E-deficient mice,” Am J Clin Nutr., vol. 71, pp. 1062-1076, 2000.

[5] P. V. Babu, K. E. Sabitha, and C. S. Shyamaladevi, “Therapeutic effect of green tea extract on oxidative stress in aorta and heart of streptozotocin diabetic rats,” Chem Biol Interact., vol. 162, pp. 114-120, 2006.

[6] J. Bruneton, Pharmacognosy. phytochemistry. Plantes Medicinales Paris: Technique Documentation-Lavoisier, 2001.

[7] J. Bernatoniene, “Kopustinskiene, D.M. The Role of Catechins in Cellular Responses,” Molecular, vol. 23, 2018, Art. no. 965.

[8] M. Farhan, “Green Tea Catechins: Nature’s Way of Preventing and Treating Cancer,” Int. J. Mol. Sci., vol. 23, 2022, Art. no. 10713.

[9] Y. Han, Z. Cheng, Y. Zhang, N. Zhang, X. Zhu, X. Chen, Y. Shao, Y. Cheng, C. Wang, Y. Luo et al., “Effect of Metal Ions and PH on the Emulsifying Properties of Polysaccharide Conjugates Prepared from Low-Grade Green Tea,” Food Hydrocoll, vol. 102, 2020, Art. no. 105624.

[10] M. Farhan, A. Zafar, S. Chibber, H. Y. Khan, H. Arif, and S. M. Hadi, “Mobilization of copper ions in human peripheral lymphocytes by catechins leading to oxidative DNA breakage: A structure activity study. Arch,” Biochem. Biophys, vol. 580, pp. 31-40, 2015.

[11] M. Farhan, H. Y. Khan, M. Oves, A. Al-Harrasi, N. Rehmani, H. Arif, S. M. Hadi, and A. Ahmad, “Cancer therapy by catechins involves redox cycling of copper ions and generation of reactive oxygen species,” Toxins, vol. 8, 2016, Art. no. 37.

[12] S. Wu, W. Zhu, P. Thompson, and Y. A. Hannun, “Evaluating Intrinsic and Non-Intrinsic Cancer Risk Factors,” Nat. Commun., vol. 9, 2018, Art. no. 3490.

[13] WHO, “Cancer Key Facts”. [Online]. Available: https://www.who.int/news-room/fact-sheets/detail/cancer. [Accessed Jan. 03, 2023].

[14] J. Ferlay, M. Colombet, I. Soerjomataram, C. Mathers, D. M. Parkin, M. Piñeros, A. Znaor, and F. Bray, “Estimating the Global Cancer Incidence and Mortality in 2018: GLOBOCAN Sources and Methods,” Int. J. Cancer, vol. 144, pp. 1941-1953, 2019.

[15] J. -W. Oh, M. Muthu, S. S. C. Pushparaj, and J. Gopal, “Anticancer Therapeutic Effects of Green Tea Catechins (GTCs) When Integrated with Antioxidant Natural Components,” Molecules, vol. 28, 2023, Art. no. 2151.

[16] M. Graille, P. Wild, J. J. Sauvain, M. Hemmendinger, I. Guseva Canu, and N. B. Hopf, “Urinary 8-OHdG as a Biomarker for Oxidative Stress: A Systematic Literature Review and Meta-Analysis,” Int. J. Mol. Sci., vol. 21, 2020, Art. no. 3743.

[17] M. N. Najafi, M. Salehi, M. Ghazanfarpour, Z. S. Hoseini, and M. Khadem-Rezaiyan, “The Association between Green Tea Consumption and Breast Cancer Risk: A Systematic Review and Meta-Analysis,” Phytother. Res., vol. 32, pp. 1855-1864, 2018.

[18] L. Zhang, C. -T. Ho, J. Zhou, J. S. Santos, L. Armstrong, and D. Granato, “Chemistry and Biological Activities of Processed Camellia sinensis Teas: A Comprehensive Review. Compr,” Rev. Food Sci. Food Saf., vol. 18, pp. 474-1495, 2019.

[19] X. Chen, Y. Du, L. Wu, J. Xie, X. Chen, B. Hu, Z. Wu, Q. Yao, and Q. Li, “Effects of Tea-Polysaccharide Conjugates and Metal Ions on Precipitate Formation by Epigallocatechin Gallate and Caffeine, the Key Components of Green Tea Infusion,” J. Agric. Food Chem., vol. 67, pp. 3744-3751, 2019.

[20] Y. Shirakami and M. Shimizu, “Possible Mechanisms of Green Tea and Its Constituents against Cancer,” Molecules, vol. 23, 2018, Art. no. 2284.

[21] T. A. Nguyen and T. H. Bui, “Study on Flavonoid and Coumarin components of drugs in dispersion method,” Journal of Pharmacy, vol. 368, pp. 37-40, 2008.

[22] B. Mahesh and S. Satish, “Antimicrobial activity of some important medicinal plant against plant and human pathogens,” World J. Agric. Sci., vol. 4, no. S, pp. 839-843, 2008.

[23] H. Abramovič, B. Grobin, N. P. Ulrih, and B. Cigić, “Relevance and Standardization of In Vitro Antioxidant Assays: ABTS, DPPH, and Folin–Ciocalteu,” Journal of Chemistry, vol. 2018, pp. 1-9, 2018.

[24] P. Skehan, R. Storeng, D. Scudiero, A. Monks, J. McMahon, D. Vistica, J. T. Warren, H. Bokesch, S. Kenney, and M. R. Boyd, “New colorimetric cytotoxic assay for anticancer-drug screening,” Journal of the National Cancer Institute, vol. 82, no. 13, pp. 1107-1112, 1990.

[25] S. Wang, R. Chen, Z. Zhong, Z. Shi, M. Chen, and Y. Wang, “Epigallocatechin-3-Gallate Potentiates the Effect of Curcumin in Inducing Growth Inhibition and Apoptosis of Resistant Breast Cancer Cells,” Am. J. Chin. Med., vol. 42, pp. 1279-1300, 2014.

DOI: https://doi.org/10.34238/tnu-jst.9363

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