DETERMINATION OF CURCUMIN CONTENT OF SOME PRODUCTS  FROM TURMERIC STARCH IN DAK LAK  USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Huỳnh Văn Chung; Trần Lưu Phúc; Hà Hoàng Anh Vĩnh; Huỳnh Thị Như Quỳnh; Lê Trung Khoảng; Hoàng Thị Thu Huyền; Vũ Thị Hồng Nhung; Nguyễn Quốc Thông

doi:10.34238/tnu-jst.9369

DETERMINATION OF CURCUMIN CONTENT OF SOME PRODUCTS FROM TURMERIC STARCH IN DAK LAK USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

About this article

Received: 08/12/23 Revised: 05/03/24 Published: 11/03/24

Authors

1. Huynh Van Chung , Buon Ma Thuot Medical University
2. Tran Luu Phuc, Buon Ma Thuot Medical University
3. Ha Hoang Anh Vinh, Buon Ma Thuot Medical University
4. Huynh Thi Nhu Quynh, Buon Ma Thuot Medical University
5. Le Trung Khoang, Buon Ma Thuot Medical University
6. Hoang Thi Thu Huyen, Buon Ma Thuot Medical University
7. Vu Thi Hong Nhung, Buon Ma Thuot Medical University
8. Nguyen Quoc Thong, Ba Ria Vung Tau Medical College

Abstract

This study was conducted with the purpose of evaluating curcumin content in some turmeric starch products circulating in Dak Lak using high performance liquid chromatography (HPLC) with PDA detector. The quantification process was carried out on Water's e2695 system using a 5µm C18 column (4.6x250 mm), the mobile phase used was acetonitrile - methanol- acetic acid 2% (60:20:20, v/v/v) at a solvent rate of 1.0 ml/min and measured at a wavelength of 426 nm. The analytical method has been evaluated according to ICH regulations with a linear range 0.01-300 µg/ml, limit of detection (LOD) 0.01 µg/ml, limit of quantification (LOQ) 0.033 µg/ml. The quantitative method achieves accuracy through recovery from 98.8 - 100.7%, good repeatability with relative standard deviation of retention time and peak area of 0.072% and 1.164%, respectively. The study was conducted on 16 samples of turmeric powder and 4 samples of turmeric granules circulating in Dak Lak.

Keywords

Curcumin; Turmeric starch; HPLC; Determination; Dak Lak

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References

[1] B. Aggarwal, Y.-J. Surh, and S. Shishodia, The Molecular Targets and Therapeutic Uses of Curcumin in Health and Disease, Advances in Experimental Medicine and Biology, 2007.

[2] P. R. Holt, S. Katz, and R. Kirshoff, "Curcumin therapy in inflammatory bowel disease: a pilot study," Digestive diseases and sciences, vol. 50, no. 11, pp. 2191-2193, 2005.

[3] S. Shishodia, G. Sethi, and B. B. Aggarwal, "Curcumin: getting back to the roots," Annals of the New York Academy of Sciences, vol. 1056, pp. 206-217, 2005.

[4] R. A. Sharma, A. J. Gescher, and W. P. Steward, “Curcumin: the story so far,” European Journal of cancer Oxford, England, vol. 41, no. 13, pp. 1955-1968, 2005.

[5] N. Chainani-Wu, "Safety and anti-inflammatory activity of curcumin: a component of tumeric (Curcuma longa)," Journal of alternative and complementary medicine (New York, N.Y.), vol. 9, no. 1, pp. 161-168, 2003.

[6] M. Iqbal, S. D. Sharma, Y. Okazaki et al., "Dietary supplementation of curcumin enhances antioxidant and phase II metabolizing enzymes in ddY male mice: possible role in protection against chemical carcinogenesis and toxicity," Pharmacology & toxicology, vol. 92, no. 1, pp. 33-38, 2003.

[7] M. C. Ramírez-Tortosa, M. D. Mesa, M. C. Aguilera et al., "Oral administration of a turmeric extract inhibits LDL oxidation and has hypocholesterolemic effects in rabbits with experimental atherosclerosis," Atherosclerosis, vol. 147, no. 2, pp. 371-378, 1999.

[8] P. S. Babu and K. Srinivasan, "Hypolipidemic action of curcumin, the active principle of turmeric (Curcuma longa) in streptozotocin induced diabetic rats," Molecular and cellular biochemistry, vol. 166, no. 1-2, pp. 169-175, 1997.

[9] K.B. Soni and R. Kuttan, "Effect of oral curcumin administration on serum peroxides and cholesterol levels in human volunteers," Indian Journal of physiology and pharmacology, vol. 36, no. 4, pp. 273-275, 1992.

[10] A. Duvoix, R. Blasius, S. Delhalle et al., "Chemopreventive and therapeutic effects of curcumin," Cancer letters, vol. 223, no. 2, pp. 181-190, 2005.

[11] G. Garcea, D. P. Berry, D. J. Jones et al., "Consumption of the putative chemopreventive agent curcumin by cancer patients: assessment of curcumin levels in the colorectum and their pharmacodynamic consequences," Cancer epidemiology, biomarkers & prevention: a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, vol. 14, no. 1, pp. 120-125, 2005.

[12] T. H. Leu and M. C. Maa, "The molecular mechanisms for the antitumorigenic effect of curcumin," Current medicinal chemistry. Anti-cancer agents, vol. 2, no. 3, pp. 357-370, 2002.

[13] Ministry of Health, Pharmacopoeia vietnamica, 2018.

[14] N. Núñez, O. Vidal-Casanella, S. Sentellas et al., "Characterization, Classification and Authentication of Turmeric and Curry Samples by Targeted LC-HRMS Polyphenolic and Curcuminoid Profiling and Chemometrics," Molecules (Basel, Switzerland), vol. 25, no. 12, pp. 1-16, 2020.

[15] T. N. N. Le, T. N. H. Nguyen, and T. T. H. Tran, “Preliminary investigation of curcumin contents in the rhizome of the yellow turmeric (curcuma longa l. zingiberaceae) according to cultivated regions by hplc method,” Journal of science of Lac Hong university, vol. 12, pp. 029-032, 2021.

[16] ICH, Harmonised Tripartite Guideline: Guidelines for validation of analytical procedures: Q2 (R1). Text and Methodology, 2005.

[17] A. H. Antunes, J. F. Mota et al., "Bioanalytical method by HPLC-FLD for curcumin analysis in supplemented athletes," Saudi Pharm J., vol. 28, no. 5, pp. 559-606, 2020.

[18] M. Kazemi, J. Emami, F. Hasanzadeh et al., "Development of a RP-HPLC method for analysis of docetaxel in tumor-bearing mice plasma and tissues following injection of docetaxel-loaded pH responsive targeting polymeric micelles," Research in pharmaceutical sciences, vol. 15, no. 1, pp. 1-13, 2020.

[19] J. C. Miller and J. N. Miller, Statistics and Chemometrics for Analytical Chemistry, sixth edition, Pearson Education, 2010.

[20] AOAC, Appendix K: Guidelines for Dietary Supplements and Botanicals, Section 3.4.1- 3.4.2, 2013.

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DOI: https://doi.org/10.34238/tnu-jst.9369

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