ASSOCIATION BETWEEN MTHFR C677T POLYMORPHISM  AND FOLATE STATUS IN PREGNANT WOMEN WITH  GESTATIONAL DIABETES MELLITUS AT CAN THO MATERNITY HOSPITAL

Phạm Thị Ngọc Nga; Huỳnh Thị Ngọc Châu

doi:10.34238/tnu-jst.12157

ASSOCIATION BETWEEN MTHFR C677T POLYMORPHISM AND FOLATE STATUS IN PREGNANT WOMEN WITH GESTATIONAL DIABETES MELLITUS AT CAN THO MATERNITY HOSPITAL

About this article

Received: 28/02/25 Revised: 27/03/25 Published: 28/03/25

Authors

1. Pham Thi Ngoc Nga , Can Tho University of Medicine and Pharmacy
2. Huynh Thi Ngoc Chau, Can Tho University of Medicine and Pharmacy

Abstract

Gestational diabetes mellitus is the most common metabolic disorder during pregnancy, with a rising prevalence worldwide, including in Vietnam. Folic acid is essential in pregnancy, and the MTHFR C677T (rs1801133) polymorphism significantly influences folate metabolism. This study investigates the MTHFR C677T polymorphism and folate status in 96 pregnant women with gestational diabetes mellitus at Can Tho Maternity Hospital from June 2023 to June 2024. Among the participants, 63.5% were under 35 years old, while 36.5% were 35 years or older. The MTHFR C677T genotype distribution was CC (65.6%), CT (31.3%), and TT (3.1%), with allele frequencies of C (81.2%) and T (18.8%). The mean folate concentration was 20.94 ± 7.63 ng/mL, with 57.3% having normal folate levels and 42.7% having elevated levels. A significant association was found between folate status and the CT genotype, allele frequencies, and genotype models of the MTHFR C677T polymorphism. The presence of the T allele may lead to altered folate metabolism, which in turn could influence insulin resistance and increase the risk of gestational diabetes mellitus. The findings highlight the importance of genetic screening and personalized folate supplementation strategies in pregnant women with gestational diabetes mellitus gestational diabetes mellitus. Larger case-control studies are needed to better understand the relationship between MTHFR C677T polymorphism, folate metabolism, and gestational diabetes mellitusrisk.

Keywords

Folate; Gestational diabetes mellitus; MTHFR C677T polymorphism; Pregnancy; Vitamin B9

Full Text:

PDF

References

[1] Ministry of Health, Decision No. 1470/QD-BYT, National Guidelines for Screening and Management of Gestational Diabetes Mellitus, 2024.

[2] Q. Li et al., "High-Dose Folic Acid Supplement Use From Prepregnancy Through Midpregnancy Is Associated With Increased Risk of Gestational Diabetes Mellitus: A Prospective Cohort Study," Diabetes Care, vol. 42, no. 7, pp. e113-e115, 2019, doi: 10.2337/dc18-2572.

[3] G. D. Cianni, E. Lacaria, C. Lencioni, and V. Resi, "Preventing type 2 diabetes and cardiovascular disease in women with gestational diabetes - The evidence and potential strategies," Diabetes Res. Clin. Pract., vol. 145, pp. 184-192, 2018, doi: 10.1016/j.diabres.2018.04.021.

[4] M. Hod et al., "The International Federation of Gynecology and Obstetrics (FIGO) Initiative on gestational diabetes mellitus: A pragmatic guide for diagnosis, management, and care," International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics, vol. 131, pp. S173-S211, 2015.

[5] W. W. Zhu, H. X. Yang, C. Wang, R. N. Su, H. Feng, and A. Kapur, "High Prevalence of Gestational Diabetes Mellitus in Beijing: Effect of Maternal Birth Weight and Other Risk Factors," Chin. Med. J. (Engl), vol. 130, no. 9, pp. 1019-1025, 2017, doi: 10.4103/0366-6999.204930.

[6] K. R. Patel and A. Sobczynska-Malefora, "The adverse effects of an excessive folic acid intake," Eur. J. Clin. Nutr., vol. 71, no. 2, pp. 159-163, 2017, doi: 10.1038/ejcn.2016.194.

[7] C. S. Yajnik et al., "Vitamin B 12 and folate concentrations during pregnancy and insulin resistance in the offspring: the Pune Maternal Nutrition Study," Diabetologia, vol. 51, pp. 29-38, 2008.

[8] J. S. Lai et al., "High folate and low vitamin B12 status during pregnancy is associated with gestational diabetes mellitus," Clin. Nutr., vol. 37, no. 3, pp. 940-947, 2018, doi: 10.1016/j.clnu.2017.03.022.

[9] B. Zhu et al., "Folic Acid Supplement Intake in Early Pregnancy Increases Risk of Gestational Diabetes Mellitus: Evidence From a Prospective Cohort Study," Diabetes Care, vol. 39, no. 3, 2016, doi: 10.2337/dc15-2389.

[10] M. Desai and J. B. Chauhan, "Computational analysis for the determination of deleterious nsSNPs in human MTHFR gene," Comput. Biol. Chem., vol. 74, pp. 20-30, 2018, doi: 10.1016/j.compbiolchem.2018.02.022.

[11] C. J. Boushey, S. A. Beresford, G. S. Omenn, and A. G. Motulsky, "A quantitative assessment of plasma homocysteine as a risk factor for vascular disease. Probable benefits of increasing folic acid intakes," JAMA, vol. 274, no. 13, pp. 1049-1057, 1995, doi: 10.1001/jama.1995.03530130055028.

[12] F. Li et al., "Analysis of risk factors related to gestational diabetes mellitus," Taiwan J. Obstet. Gynecol., vol. 59, no. 5, pp. 718-722, 2020, doi: 10.1016/j.tjog.2020.07.016.

[13] M. Visentin, N. Diop-Bove, R. Zhao, and I. D. Goldman, "The intestinal absorption of folates," Annu. Rev. Physiol., vol. 76, no. 1, pp. 251-274, 2014, doi: 10.1146/annurev-physiol-020911-153251.

[14] M. Abbassi-Ghanavati, L. G. Greer, and F. G. Cunningham, "Pregnancy and laboratory studies: a reference table for clinicians," Obstet. Gynecol., vol. 114, no. 6, pp. 1326-1331, 2009, doi: 10.1097/AOG.0b013e3181c2bde8.

[15] T. T. Que, "Study on the Relationship Between Serum Folate, Homocysteine Levels, and Some MTHFR Gene Polymorphisms in Women with Reproductive Abnormalities," Doctoral Thesis, Hanoi Medical University, Hanoi, Vietnam, 2021.

[16] U. Yadav, P. Kumar, S. Gupta, and V. Rai, "Distribution of MTHFR C677T Gene Polymorphism in Healthy North Indian Population and an Updated Meta-analysis," Indian J. Clin. Biochem., vol. 32, no. 4, pp. 399-410, 2017, doi: 10.1007/s12291-016-0619-0.

[17] P. J. Liu et al., "Associations Between Gestational Diabetes Mellitus Risk and Folate Status in Early Pregnancy and MTHFR C677T Polymorphisms in Chinese Women," Diabetes Metab. Syndr. Obes., vol. 13, pp. 1499-1507, 2020, doi: 10.2147/DMSO.S250279.

[18] J. Ni et al., "Association between the MTHFR C677T polymorphism, blood folate and vitamin B12 deficiency, and elevated serum total homocysteine in healthy individuals in Yunnan Province, China," J. Chin. Med. Assoc., vol. 80, no. 3, pp. 147-153, 2017, doi: 10.1016/j.jcma.2016.07.005.

[19] Z. Lin et al., "Interactions between genetic variants involved in the folate metabolic pathway and serum lipid, homocysteine levels on the risk of recurrent spontaneous abortion," Lipids Health Dis., vol. 18, no. 1, pp. 1-13, 2019, doi: 10.1186/s12944-019-1083-7.

[20] R. Kedar and D. Chandel, "MTHFR gene polymorphism and associated nutritional deficiency in the etiology and pathogenesis of Down syndrome," Egyptian Journal of Medical Human Genetics, vol. 20, pp. 1-10, 2019.

[21] U. Pellecchia, R. Crestani, T. Decroo, R. V. den Bergh, and Y. Al-Kourdi, "Social Consequences of Ebola Containment Measures in Liberia," PLoS One, vol. 10, no. 12, 2015, doi: 10.1371/journal.pone.0143036.

"Times New Roman",serif;mso-fareast-font-family:Calibri;mso-fareast-theme-font:

minor-latin;mso-bidi-theme-font:minor-bidi;mso-ansi-language:PT-BR;mso-fareast-language:

EN-US;mso-bidi-language:AR-SA'>

DOI: https://doi.org/10.34238/tnu-jst.12157

Refbacks

There are currently no refbacks.



Remember me