UV-VIS SPECTROPHOTOMETRY FOR QUANTITATIVE DETERMINATION OF THE TOTAL PHENOLIC COMPOUNDS FROM LEAVES  OF MORINGA OLEIFERA

Nguyễn Khánh Thùy Linh; Võ Thị Quỳnh Nhi; Thái Thị Thu Nhiên; Huỳnh Thị Diệu Hân; Lê Thị Hồng Hà; Nguyễn Thị Yến Vi

UV-VIS SPECTROPHOTOMETRY FOR QUANTITATIVE DETERMINATION OF THE TOTAL PHENOLIC COMPOUNDS FROM LEAVES OF MORINGA OLEIFERA

About this article

Received: 13/08/19 Revised: 18/10/19 Published: 21/10/19

Authors

1. Nguyen Khanh Thuy Linh , Hue University of Medicine and Pharmacy
2. Vo Thi Quynh Nhi, Hue University of Medicine and Pharmacy
3. Thai Thi Thu Nhien, Hue University of Medicine and Pharmacy
4. Huynh Thi Dieu Han, Hue University of Medicine and Pharmacy
5. Le Thi Hong Ha, Hue University of Medicine and Pharmacy
6. Nguyen Thi Yen Vi, Hue University of Medicine and Pharmacy

Abstract

Background: Moringa oleifera has many valuable biological effects. Polyphenol compounds play an important role in preventing the harmful effects of oxidative stress. It is necessary to develop and validate a quantitative analysis procedure of total polyphenols in the leaves of Moringa oleifera using UV-VS method to control the quality of Moringa oleifera. Material and Methods: leaves of Moringa oleifera are collected in Hue city. Determining total polyphenols using UV-VIS method based on reaction between polyphenols and folin-ciocalteu reagent; validate this procedure according to AOAC guidelines. Results: The quantitative analysis was performed by reaction between total polyphenols and folin-ciocalteu reagent with detective wavelength of 765 nm. The method was validated to have satisfied linearity in the range of 10-50 mg/ml. Intra-day and inter-day precision tests showed the RSD% < 2% and desirable accuracy (revovery 98.79% - 101.93%). Conclusion: The quantitative analysis procedure of total polyphenols from Moringa oleifera using UV-VIS method was validated.

Keywords

Pharmacognosy, Moringa oleifera; UV-VIS; total polyphenols

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