SPECIES IDENTIFICATION OF LEPTOBOTIA PELLEGRINI FANG,  1936 IN NGHE AN USING DNA BARCODE

Lê Đình Chắc; Hoàng Ngọc Thảo; Nguyễn Thị Yến; Ông Vĩnh An; Lê Anh Sơn

doi:10.34238/tnu-jst.9016

SPECIES IDENTIFICATION OF LEPTOBOTIA PELLEGRINI FANG, 1936 IN NGHE AN USING DNA BARCODE

About this article

Received: 22/10/23 Revised: 29/11/23 Published: 01/12/23

Authors

1. Le Dinh Chac , Hong Duc University
2. Hoang Ngoc Thao, Hong Duc University
3. Nguyen Thi Yen, Hong Duc University
4. ong Vinh An, Vinh University
5. Le Anh Son, Thanh Hoa Department of Education and Training

Abstract

Leptobotia pellegrini Fang, 1936, is an ornamental and high commercial value species. In Vietnam, this species was recorded in 2005 by Nguyen Van Hao in Hong River with the name Leptobotia elongata, and was re-identified as Leptobotia pellegrini (Kottelat: 2012, 2013). In 2022, Hoang et al. Discovered this species in Tuong Duong, Nghe An, described ist morphological characteristics, and identified this as a new distribution area of L. pellegrini in Vietnam. In this study, we used molecular taxonomy research methods to obtain ba DNA barcode loci: Cybt, ND2 and 16S to identify L. pellegrini in Vietnam. The sizes of sequences of the Cybt, ND2, and 16S we obtained respectively are Cytb 818 bp, ND2 512 bp and 16S 750 bp. Samples of the same species, L. pellegrini, are placed in the same group on the phylogenetic tree with high bootstrap coefficients, from 99%, 100%, and 89% based on Cytb, ND2 and 16S gene sequences. This has contributed to the analysis, description of morphological characteristics and new records of L. pellegrini species in Vietnam.

Keywords

Cybt; ND2; 16S; Leptobotia pellegrini; Loach

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DOI: https://doi.org/10.34238/tnu-jst.9016

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