ISOLATION AND CHARACTERIZATION OF A LYTIC BACTERIOPHAGE C1 SPECIFIC TO THE MARINE BACTERIUM VIBRIO PARAHAEMOLYTICUS IN QUANG NINH PROVINCE

Ngô Thị Huyền Trang; Nguyễn Thị Thanh Ngọc; Nguyễn Thị Thu An; Nguyễn Văn Thế; Vũ Thị Hạnh Nguyên; Phí Quyết Tiến; Lương Hùng Tiến; Quách Ngọc Tùng

doi:10.34238/tnu-jst.10081

ISOLATION AND CHARACTERIZATION OF A LYTIC BACTERIOPHAGE C1 SPECIFIC TO THE MARINE BACTERIUM VIBRIO PARAHAEMOLYTICUS IN QUANG NINH PROVINCE

About this article

Received: 09/04/24 Revised: 26/06/24 Published: 26/06/24

Authors

1. Ngo Thi Huyen Trang , Institute of Biotechnology – VAST
2. Nguyen Thi Thanh Ngoc, East Asia University of Technology
3. Nguyen Thi Thu An, Institute of Biotechnology – VAST
4. Nguyen Van The, Institute of Biotechnology – VAST
5. Vu Thi Hanh Nguyen, Institute of Biotechnology – VAST
6. Phi Quyet Tien, Institute of Biotechnology – VAST
7. Luong Hung Tien, TNU - University of Agriculture and Forestry
8. Quach Ngoc Tung, Institute of Biotechnology – VAST

Abstract

Acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus is the main cause leading to economic losses to shrimp farming. In addition, the emergence of multiple drug-resistant Vibriohas underscored the urgent need for alternative strategies such as phage therapy. In the present study, 7 bacteriophages specific to Vibrio spp. were isolated from water and sludge samples collected at the shrimp farms in Quang Ninh province. Among them, phage C1 was highly effetive in lysing Vibrio panurili A1.2, Vibrio alginolyticus A2, V. alginolyticus D1, and especially V. parahaemolyticus H3. Using transmission electron microscopy, the phage C1 was observed to belongto the Podoviridae family with a head diameter of 52.73 nm and a very short tail. Phage C1 exhibited the highest titer (9.6±0.12 log PFU/mL) at an optimal MOI ratio of 0.0001. Assessement of lysis potential revealed that phage C1 was able to maintain strong lytic activity for 10 hours. Notably, C1 had a brief latent phase (<10 min) with a high burst size of 294 PFU/mL and resilience to a decent range of temperatures (20-50°C) and pHs (5-11). These findings further enhance our understanding of biological characteristics Vibrio phage from local shrimp farms and its potential as a biocontrol agent for vibriosis included by Vibrio spp.

Keywords

Bacteriophage; Lytic activity; Phage therapy; Vibriosis; Vibrio parahaemolyticus

Full Text:

PDF

References

[1] T. Manchanayake, A. Salleh, M. N. A. Amal, I. S. M. Yasin, and M. Zamri-Saad, "Pathology and pathogenesis of Vibrio infection in fish: A review," Aquaculture Reports, vol. 28, 2023, Art. no. 101459.

[2] M. Y. Ina-Salwany, N. Al-Saari et al., "Vibriosis in fish: A review on disease development and prevention," Journal of Aquatic Animal Health, vol. 31, no. 1, pp. 3-22, 2019.

[3] K. A. Brossard Stoos, J. Ren et al., "Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types," Frontiers in Microbiology, Original Research, vol. 13,2022, Art. no. 1041942.

[4] Y. P. Yu, T. Gong, G. Jost, W. H. Liu, D. Z. Ye, and Z. H. Luo, "Isolation and characterization of five lytic bacteriophages infecting a Vibrio strain closely related to Vibrio owensii," FEMS Microbiology Letters, vol. 348, no. 2, pp. 112-119, 2013.

[5] F. L. Gordillo Altamirano and J. J. Barr, "Phage therapy in the postantibiotic era," Clinical Microbiology Reviews, vol. 32, no. 2, 2019, Art. no. e00066-18.

[6] S. Dubey, A. Singh, B. T. N. Kumar, N. K. Singh, and A. Tyagi, "Isolation and characterization of bacteriophages from inland saline aquaculture environments to control Vibrio parahaemolyticus contamination in shrimp," Indian Journal of Microbiology, vol. 61, no. 2, pp. 212-217, 2021.

[7] Q. Hao, Y. Bai et al., "Isolation and characterization of bacteriophage VA5 against Vibrio alginolyticus," Microorganisms, vol. 11, no. 12, 2023, Art. no. 2822.

[8] T. Van, K. Do Tan et al., "Effect of bacteriophages and chamber bitter (Phyllanthus amarus) in combination on Vibrio parahaemolyticus," Journal of Applied Biology & Biotechnology, vol. 11, no. 3, pp. 70-76, 2023.

[9] A. M. Kropinski, A. Mazzocco, T. E. Waddell, E. Lingohr, and R. P. Johnson, "Enumeration of bacteriophages by double agar overlay plaque assay," Methods in Molecular Biology, vol. 501, pp. 69-76, 2009.

[10] C. W. Tan, Y. Rukayadi et al., "Isolation and characterization of six Vibrio parahaemolyticus lytic bacteriophages from seafood samples," Frontiers in Microbiology, Original Research, vol. 12, 2021, Art. no. 616548.

[11] J. Fu, Y. Li, L. Zhao, C. Wu, and Z. He, "Characterization of vB_ValM_PVA8, a broad-host-range bacteriophage infecting Vibrio alginolyticus and Vibrio parahaemolyticus," Frontiers in Microbiology, Original Research, vol. 14, 2023, Art. no. 1105924.

[12] H.-J. Chang, J. Hong et al., "Growth inhibitory effect of bacteriophages isolated from western and southern coastal areas of Korea against Vibrio parahaemolyticus in Manila clams," Applied Biological Chemistry, vol. 59, no. 3, pp. 359-365, 2016.

[13] N. Stalin and P. Srinivasan, "Efficacy of potential phage cocktails against Vibrio harveyi and closely related Vibrio species isolated from shrimp aquaculture environment in the south east coast of India," Veterinary Microbiology, vol. 207, pp. 83-96, 2017.

[14] Y. Chen, W. Li, K. Shi, Z. Fang, Y. Yang, and R. Zhang, "Isolation and characterization of a novel phage belonging to a new genus against Vibrio parahaemolyticus," Virology Journal, vol. 20, no. 1, pp. 81, 2023.

[15] Y. Ye, H. Chen et al., "Characterization and genomic analysis of novel Vibrio parahaemolyticus phage vB_VpaP_DE10," Viruses, vol. 14, no. 8, 2022. Art. no. 1609.

[16] P. J. Walker, S. G. Siddell et al., "Changes to virus taxonomy and the Statutes ratified by the International Committee on Taxonomy of Viruses (2020)," Archives of Virology, vol. 165, no. 11, pp. 2737-2748, 2020.

[17] X. Liang, Y. Wang, B. Hong, Y. Li, Y. Ma, and J. Wang, "Isolation and characterization of a lytic Vibrio parahaemolyticus phage vB_VpaP_GHSM17 from sewage samples," Viruses, vol. 14, no. 8, 2022, Art. no. 1601.

[18] M. Yang, H. Chen et al., "Characterization and genome analysis of a novel Vibrio parahaemolyticus phage vB_VpP_DE17," Virus Research, vol. 307, 2022, Art. no. 198580.

[19] M. Yang, Y. Liang et al., "Isolation and characterization of the novel phages vB_VpS_BA3 and vB_VpS_CA8 for lysing Vibrio parahaemolyticus," Frontier in Microbiology, vol. 11, p. 259, 2020.

DOI: https://doi.org/10.34238/tnu-jst.10081

Refbacks

There are currently no refbacks.



Remember me