CHARACTERISTICS OF BARCODE NUCLEOTIDE SEQUENCES matK AND ITS OF THE Anoectochilus roxburghii (Wall.) Wall. ex Lindl. 1840 PLANTS COLLECTED IN LUANG NAMTHA PROVINCE OF LAOS

Phạm Thị Thanh Nhàn; SYSOMEPHONE Tangmany

doi:10.34238/tnu-jst.10291

CHARACTERISTICS OF BARCODE NUCLEOTIDE SEQUENCES matK AND ITS OF THE Anoectochilus roxburghii (Wall.) Wall. ex Lindl. 1840 PLANTS COLLECTED IN LUANG NAMTHA PROVINCE OF LAOS

About this article

Received: 05/05/24 Revised: 11/07/24 Published: 12/07/24

Authors

1. Pham Thi Thanh Nhan , TNU - University of Education
2. SYSOMEPHONE Tangmany, Research Institute for Educational Sciences - Ministry of Education and Sports, Laos

Abstract

The Anoectochilus roxburghii (Wall.) Wall. ex Lindl. 1840 plants have been over-exploited and recorded in Vietnam's Red Data Book. In this paper, we analyze characteristics of matK, ITS nucleotide sequences from this species collected in Luang Namtha province of Laos to find out the differences at the molecular level among species, and provide information about barcodes for the identification, exploitation and conservation of their genetic resources. Lengths of the isolated matK gene segment and ITS region are around 900bp and 500bp, respectively. Their sequences have the similarity with other species in the genus Anoectochilus, 100% and 98%, respectively, to Anoectochilus roxburghii and Anoectochilus burmannicus species in the Genbank. There are 15 positions of nucleotide changes in each gene segment. The matK and ITS barcodes can be used for detecting the genus Anoectochilus. In which, the ITS barcode identified the Anoectochilus roxburghii (Wall.) Wall. ex Lindl. 1840 species and the Anoectochilus roxburghii species with the sequence ID in NCBI of MK451732.1 are in the same branch of the phylogenetic with a bootstrap value of 98%.

Keywords

DNA barcode; ITS; Anoectochilus roxburghii (Wall.) Wall. ex Lindl. 1840; Laos; matK

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DOI: https://doi.org/10.34238/tnu-jst.10291

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