PRODUCTION AND BIOACTIVITY ASSESSMENT OF  EXOPOLYSACCHARIDE PRODUCED BY LEVILACTOBACILLUS BREVIS MT8

Nguyễn Quang Huy; Quách Ngọc Tùng; Vũ Thị Hạnh Nguyên; Nguyễn Hữu Quân; Phí Quyết Tiến

doi:10.34238/tnu-jst.10375

PRODUCTION AND BIOACTIVITY ASSESSMENT OF EXOPOLYSACCHARIDE PRODUCED BY LEVILACTOBACILLUS BREVIS MT8

About this article

Received: 13/05/24 Revised: 10/06/24 Published: 13/06/24

Authors

1. Nguyen Quang Huy, University of Science and Technology of Hanoi – VAST
2. Quach Ngoc Tung, Institute of Biotechnology – VAST
3. Vu Thi Hanh Nguyen, Institute of Biotechnology – VAST
4. Nguyen Huu Quan, TNU - University of Education
5. Phi Quyet Tien , Institute of Biotechnology – VAST

Abstract

Expolysaccharides produced by lactic acid bacteria have gained great interest for their potential applications in food, pharmaceutical and biotechnological industries due to their biological and rheological properties. In Vietnam, a few studies have explored production and bioactivity of lactic acid bacteria-derived EPS. The present study reports the improvement of EPS production by Levilactobacillus brevis MT8, in addition to assessing its antioxidant and antibacterial activities. The isolate MT8 from fermented eggplant, showing mucoid phenotype, was identified as L. brevis using 16S rRNA gene analysis. After optimization, the strain MT8 produced the maximum EPS yield of 725.7±50.1 mg/L when cultivated in the MRS medium for 48 h at 37°C, in which 20 g/L glucose was replaced by 40 g/L sucrose. Antioxidant assay revealed that the obtained EPS exhibited the highest scavenging activities against 1,1-diphemy 1-2-picryl-hydrazyl (78.0±0.4%) and hydroxyl (68.8±0.5%) at 2 mg/mL. Of note, 5 mg/mL EPS also showed antibacterial activity against Escherichia coli ATCC 11105 and Salmonella Typhimurium ATCC 14028 with inhibition zones of 5.9±0.4 mm and 7.5±0.2 mm, respectively. Therefore, the EPS synthesized by L. brevis MT8 shows promise for application in the food, cosmetics, biomedical industries. To the best of our knowledge, this is the first report to prove antibacterial activity of EPS produced by L. brevis.

Keywords

Antibacterial activity; Antioxidant; Expolysaccharide; Lactic acid bacteria; Levilactobacillus brevis

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References

[1] T. Goa, G. Beyene, M. Mekonnen, and K. Gorems, "Isolation and characterization of lactic acid bacteria from fermented milk produced in Jimma Town, Southwest Ethiopia, and evaluation of their antimicrobial activity against selected reference bacteria," International journal of food science, vol. 2022, 2022, Art. no. 2076021, doi: 10.1155/2022/2076021.

[2] Y. Wei, F. Li, L. Li, L. Huang, and Q. Li, "Genetic and biochemical characterization of an exopolysaccharide with in vitro antitumoral activity produced by Lactobacillus fermentum YL-11," Front Microbiol, Original Research vol. 10, 2019, doi: 10.3389/fmicb.2019.02898.

[3] A. K. Abdalla et al., "Exopolysaccharides as antimicrobial agents: mechanism and spectrum of activity," Front Microbiol, vol. 12, 2021, Art. no. 664395, doi: 10.3389/fmicb.2021.664395.

[4] B. Adebayo-Tayo and R. Fashogbon, "In vitro antioxidant, antibacterial, in vivo immunomodulatory, antitumor and hematological potential of exopolysaccharide produced by wild type and mutant Lactobacillus delbureckii subsp. bulgaricus," Heliyon, vol. 6, no. 2, Feb. 2020, Art. no. e03268, doi: 10.1016/j.heliyon.2020.e03268.

[5] M. S. Riaz Rajoka, Y. Wu, H. M. Mehwish, M. Bansal, and L. Zhao, "Lactobacillus exopolysaccharides: New perspectives on engineering strategies, physiochemical functions, and immunomodulatory effects on host health," Trends Food Sci Technol, vol. 103, pp. 36-48, 2020, doi: 10.1016/j.tifs.2020.06.003.

[6] A. Afreen, Z. Ahmed, and N. Khalid, "Optimization, fractional characterization, and antioxidant potential of exopolysaccharides from Levilactobacillus brevis NCCP 963 isolated from “kanji”," RSC Advances, 10.1039/D2RA07338B vol. 13, no. 29, pp. 19725-19737, 2023, doi: 10.1039/D2RA07338B.

[7] Q. N. Tung, T. Busche, V. V. Loi, J. Kalinowski, and H. Antelmann, "The redox-sensing MarR-type repressor HypS controls hypochlorite and antimicrobial resistance in Mycobacterium smegmatis," Free Radic Biol Med, vol. 147, pp. 252-261, 2020, doi: 10.1016/j.freeradbiomed.2019.12.032.

[8] J. Liu, X. Han, T. Zhang, K. Tian, Z. Li, and F. Luo, "Reactive oxygen species (ROS) scavenging biomaterials for anti-inflammatory diseases: from mechanism to therapy," J Hematol Oncol, vol. 16, no. 1, p. 116, 2023, doi: 10.1186/s13045-023-01512-7.

[9] L. Xiao et al., "Biosynthesis of exopolysaccharide and structural characterization by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir," Food chemistry. Molecular sciences, vol. 3, 2021, Art. no. 100054, doi: 10.1016/j.fochms.2021.100054.

[10] M. Feyereisen et al., "Comparative genome analysis of the Lactobacillus brevis species," BMC Genomics, vol. 20, no. 1, p. 416, 2019, doi: 10.1186/s12864-019-5783-1.

[11] N. T. Quach et al., "Structural and genetic insights into a poly-γ-glutamic acid with in vitro antioxidant activity of Bacillus velezensis VCN56," World journal of microbiology & biotechnology, vol. 38, no. 10, p. 173, 2022, doi: 10.1007/s11274-022-03364-8.

[12] T. H. N. Vu et al., "Fusarium foetens AQF6 isolated from Amentotaxus ynnanensis H.L.Li as a prolific source of antioxidant compounds," Appl Sci, vol. 14, no. 5, p. 2048, 2024.

[13] T. H. N. Vu et al., "Distribution, cytotoxicity, and antioxidant activity of fungal endophytes isolated from Tsuga chinensis (Franch.) Pritz. in Ha Giang province, Vietnam," Ann Microbiol, vol. 72, no. 1, p. 36, 2022, doi: 10.1186/s13213-022-01693-5.

[14] N. T. Quach et al., "Genome-guided investigation provides new insights into secondary metabolites of Streptomyces parvulus SX6 from Aegiceras corniculatum," (in eng), Polish journal of microbiology, vol. 71, no. 3, pp. 381-394, 2022, doi: 10.33073/pjm-2022-034.

[15] R. Du, L. Yu, N. Yu, W. Ping, G. Song, and J. Ge, "Characterization of exopolysaccharide produced by Levilactobacillus brevis HDE-9 and evaluation of its potential use in dairy products," Int J Biol Macromol, vol. 217, pp. 303-311, 2022, doi: 10.1016/j.ijbiomac.2022.07.057.

[16] X. Wang, S. Wang, J. Xu, B. Wu, Z. Hu, and H. Niu, "Isolation, characterization, and biopreservation of Lactobacillus brevis DN-1 to inhibit mold and remove aflatoxin B1 in peanut and sunflower cakes," Agriculture, vol. 14, no. 5, p. 698, 2024.

[17] M. Y. Imran et al., "Statistical optimization of exopolysaccharide production by Lactobacillus plantarum NTMI05 and NTMI20," Int J Biol Macromol, vol. 93, no. Pt A, pp. 731-745, Dec 2016, doi: 10.1016/j.ijbiomac.2016.09.007.

[18] N. R. Lee et al., "Improved production of poly-γ-glutamic acid by Bacillus subtilis D7 isolated from Doenjang, a Korean traditional fermented food, and its antioxidant activity," (in eng), Applied biochemistry and biotechnology, vol. 173, no. 4, pp. 918-32, Jun 2014, doi: 10.1007/s12010-014-0908-0.

[19] M. S. Riaz Rajoka et al., "Functional characterization and biotechnological potential of exopolysaccharide produced by Lactobacillus rhamnosus strains isolated from human breast milk," LWT, vol. 89, pp. 638-647, 2018, doi: 10.1016/j.lwt.2017.11.034.

[20] A. K. Abdalla et al., “Exopolysaccharides as Antimicrobial Agents: Mechanism and Spectrum of Activity,” Front Microbiol, vol. 12, 2021, Art. no. 664395, doi: 10.3389/fmicb.2021.664395.

[21] M. L. Werning et al., “Biological Functions of Exopolysaccharides from Lactic Acid Bacteria and Their Potential Benefits for Humans and Farmed Animals,” Foods, vol. 11, no. 9, 2022, Art. no. 1284, doi: 10.3390/foods11091284.

DOI: https://doi.org/10.34238/tnu-jst.10375

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