AN INVESTIGATION OF CHARACTERIZE ANTIBIOTIC RESISTANCE GENES AND VIRULENCE GENES OF ACINETOBACTER BAUMANNII USING ADVANCED NEXT-GENERATION SEQUENCING

Nghiêm Ngọc Minh; Trần Thị Hợp; Nguyễn Thị Diễm; Võ Thị Bích Thủy

doi:10.34238/tnu-jst.10771

AN INVESTIGATION OF CHARACTERIZE ANTIBIOTIC RESISTANCE GENES AND VIRULENCE GENES OF ACINETOBACTER BAUMANNII USING ADVANCED NEXT-GENERATION SEQUENCING

About this article

Received: 15/07/24 Revised: 17/10/24 Published: 18/10/24

Authors

1. Nghiem Ngoc Minh, Institute of Genome Research - Vietnam Academy of Science and Technology
2. Tran Thi Hop, Institute of Genome Research - Vietnam Academy of Science and Technology
3. Nguyen Thi Diem, Institute of Genome Research - Vietnam Academy of Science and Technology
4. Vo Thi Bich Thuy , Institute of Genome Research - Vietnam Academy of Science and Technology

Abstract

Acinetobacter baumannii is one of the common Gram-negative bacteria causing hospital-acquired infections, posing a serious global health threat due to its ability to resist multiple antibiotics and rapid reproduction. Thirty strains of Aci. baumannii resistant to antibiotics were studied using next-generation genome sequencing techniques, combined with bioinformatic analysis to identify genetic characteristics, gene groups related to pathogenicity and multidrug resistance. The research results showed 11 different sequence types (ST), in with ST2 being the most prevalent (36.67%). Among the 30 isolates, there were 28/30 (93.33%) exhibited resistance to three or more types of antibiotics, with all isolated strains resistant to ampicillin, whereas showing lower ratio resistance to ciprofloxacin, cefoxitin, and trimethoprim (3.33%). All multidrug-resistant strains in the study contained virulence genes. These findings indicate the circulation of multidrug-resistant Aci. baumannii strains in several major hospitals in Hanoi, helping doctors ensure effective treatment and reduce bacterial resistance, thereby minimizing patient mortality.

Keywords

Acinetobacter baumannii; Multidrug resistance; Antibiotic resistance genes; Virulence genes; Next-generation sequencing

Full Text:

PDF (Tiếng Việt)

References

[1] Z. K. Abbas-Al-Khafaji and Q. H. Aubais-aljelehawy, “Evaluation of antibiotic resistance and prevalence of multi-antibiotic resistant genes among Acinetobacter baumannii strains isolated from patients admitted to al-yarmouk hospital,” Cellular, Molecular and Biomedical Reports, vol. 1, no. 2, pp. 60-68, 2021.

[2] T. Hasan, C. H. Choi, and M. H. Oh, “Genes Involved in the Biosynthesis and Transport of Acinetobactin in Acinetobacter baumannii,” Genomics Inform, vol. 13, no. 1, pp. 2-6, 2015.

[3] M. R. Galac et al., “A Diverse Panel of Clinical Acinetobacter baumannii for Research and Development,” Antimicrob Agents Chemother, vol. 64, no. 10, 2020, doi: 10.1128/aac.00840-20.

[4] L. S. Tran, et al., “Research rate of carbapenem resistance of Acinetobacter baumannii at Can Tho city general hospital 2021-2022,” Vietnam Medical Journal, vol. 518, no. 2, pp. 312-316, 2022.

[5] Lien Chau and Duy Tinh, “Concerns about drug-resistant superbugs in Vietnam: Controlling cross-contamination,” Thanh Nien Newwspaper Online, 2019. [Online]. Available: https://thanhnien.vn/lo-ngai-sieu-khuan-khang-thuoc-tai-viet-nam-185869122.htm. [Accessed Aug. 20, 2024].

[6] P. T. H. Singh and A. Chakrabati, “Acinetobacter baumannii: A Brief Account of Mechanisms of Multidrug Resistance and Current and Future Therapeutic Management,” PubMed, vol. 2013, pp. 2602-2605, 2013.

[7] D. A. Mark et al., “Genomewide Analysis of Divergence of Antibiotic Resistance Determinants in Closely Related Isolates of Acinetobacter baumannii,” Antimicrobial Agents and Chemotherapy, vol. 54, no. 9, pp. 3569-3577, 2010.

[8] S. N. Kumkar et al., “Corrigendum: Diversity of resistant determinants, virulence factors, and mobile genetic elements in Acinetobacter baumannii from India: A comprehensive in silico genome analysis,” Frontiers in Cellular and Infection Microbiology, vol. 12, pp. 20, 2023.

[9] A. M. H. Federico Perez, K. M. Hujer, B. K. Decker, P. N. Rather, and R. A. Bonomo, “Global Challenge of Multidrug-Resistant Acinetobacter baumannii,” Antimicrobial Agents and Chemotherapy, vol. 51, no. 10, pp. 3471-3484, 2007.

[10] H. Esmaeilkhani et al., “In vivo validation of the immunogenicity of recombinant Baumannii Acinetobactin Utilization A protein (rBauA),” Microbial Pathogenesis, vol. 98, pp. 77-81, 2016.

[11] C. Liu et al., “Distribution of virulence-associated genes and antimicrobial susceptibility in clinical Acinetobacter baumannii isolates,” Oncotarget, vol. 9, no. 31, pp. 21663-21673, 2018.

[12] B. A. Eijkelkamp et al., “Comparative analysis of surface-exposed virulence factors of Acinetobacter baumannii,” BMC Genomics, vol. 15, no. 1, pp. 1020, 2014.

[13] Y. Zer et al., “Investigation of Virulence Genes of Nosocomial Acinetobacter baumannii Isolates,” Türk Mikrobiyoloji Cemiyeti Dergisi, vol. 54, pp. 32-39, 2024.

[14] A. Correa et al., “Distinct Genetic Diversity of Carbapenem-Resistant Acinetobacter baumannii from Colombian Hospitals,” Microb Drug Resist, vol. 24, no. 1, pp. 48-54, 2018.

[15] J. Loraine et al., “Genomic and phenotypic analyses of Acinetobacter baumannii isolates from three tertiary care hospitals in Thailand,” Journal Front Microbiol, vol. 11, p. 548, 2020.

[16] J. Moradi et al., “Antibiotic resistance of Acinetobacter baumannii in Iran: a systemic review of the published literature,” Journal Osong Public Health Res Perspect, vol. 6, no. 2, pp. 79-86, 2015.

[17] S. T. Nguyen and H. V. Pham, “Eight novel genes identified at Acinetobacter baumannii caused respiratory infection in Thong nhat Dong Nai general hospital,” Medical News, vol. 2018, pp. 66-74, 2018.

[18] T. W. Loehfelm, N. R. Luke, and A. A. Campagnari, “Identification and characterization of an Acinetobacter baumannii biofilm-associated protein,” J. Bacteriol, vol. 190, no. 3, pp. 1036-1044, 2008.

[19] S. J. Youn, K. S. Hoe, and K. Semi, “Persistence of Multidrug-Resistant Acinetobacter baumannii Isolates Harboring blaOXA-23 and bap for 5 Years,” Journal of Microbiology and Biotechnology, vol. 26, no. 8, pp. 1481-1489, 2016.

[20] S. Biglari et al., “Antimicrobial Resistance Mechanisms and Genetic Diversity of Multidrug-Resistant Acinetobacter baumannii Isolated from a Teaching Hospital in Malaysia,” Microbial Drug Resistance, vol. 23, no. 5, pp. 545-555, 2016.

font-family:"Times New Roman",serif;mso-fareast-font-family:"Times New Roman";

mso-fareast-theme-font:minor-fareast;mso-ansi-language:VI;mso-fareast-language:

EN-US;mso-bidi-language:AR-SA'>

DOI: https://doi.org/10.34238/tnu-jst.10771

Refbacks

There are currently no refbacks.



Remember me