PHYLOGENETIC ANALYSIS BASED ON psbZ-rps14 AND rbcL-accD SEQUENCES TO SUPPORT SPECIES IDENTIFICATION OF  (Hoya varticillata var varticillata)

Hoàng Việt Cường; Phạm Thị Thu Hiền; Từ Quang Tân; Chu Hoàng Mậu

doi:10.34238/tnu-jst.10996

PHYLOGENETIC ANALYSIS BASED ON psbZ-rps14 AND rbcL-accD SEQUENCES TO SUPPORT SPECIES IDENTIFICATION OF (Hoya varticillata var varticillata)

About this article

Received: 25/08/24 Revised: 17/10/24 Published: 18/10/24

Authors

1. Hoang Viet Cuong, TNU - University of Education
2. Pham Thi Thu Hien, TNU - University of Education
3. Tu Quang Tan, TNU - University of Education
4. Chu Hoang Mau , TNU - University of Education

Abstract

Hoya verticillata var. verticillata is a valuable ornamental and medicinal plant containing many biologically active substances. In traditional medicine, H. verticillata var verticillate has been used as a galactagogue to treat wounds and broken bones. H. verticillata var verticillata has a similar morphology to some species of the Hoya genus, so it is difficult to identify. When the roots, stems, and leaves have changed shape or are in powder form due to mechanical impact, it is even more difficult to distinguish. This study aims to identify DNA markers to support the identification of H. verticillata var verticillata species. Using molecular evolutionary analysis methods, based on the sequence of the space region of the chloroplast genome with high nucleotide diversity, the phylogenetic tree showing the genetic relationship between H. verticillata var verticillata and species of the Hoya genus was established. Analysis based on the psbZ-rps14 sequence showed that H. verticilata var verticillata was distributed in the same group as Hoya carnosa (NC_045868.1) and Hoya meliflua (NC_069571.1), but the bootstrap coefficients were low (76% and 51%, respectively). For the rbcL-accD sequence, H. verticilata var verticillata was very closely related to Hoya carnosa (NC_045868.1) and Hoya ovalifolia (NC_069563.1) with bootstrap coefficients of 96% and 94%, respectively. The rbcL-accD marker in the chloroplast genome is a potential DNA barcode candidate to aid in distinguishing H. verticilata var verticillata from other species in the Hoya genus.

Keywords

Chloroplast genome; Hoya verticillata; Molecular evolution; psbZ-rps14; rbcL-accD

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DOI: https://doi.org/10.34238/tnu-jst.10996

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