GENE SEQUENCE ANALYSIS of rbcL AND ndhF ISOLATED FROM  Hoya lockii FOR SPECIES IDENTIFICATION

Nguyễn Thị Thu Ngà; Nguyễn Thị Thu Hằng; Phạm Thị Ngọc Ánh; Trần Thị Hồng; Nguyễn Vũ Bão; Manivanh Yongsa; Sỹ Danh Thường; Chu Hoàng Mậu

doi:10.34238/tnu-jst.12338

GENE SEQUENCE ANALYSIS of rbcL AND ndhF ISOLATED FROM Hoya lockii FOR SPECIES IDENTIFICATION

About this article

Received: 18/03/25 Revised: 17/07/25 Published: 17/07/25

Authors

1. Nguyen Thi Thu Nga , TNU - University of Education
2. Nguyen Thi Thu Hang, TNU - University of Education
3. Pham Thi Ngoc anh, TNU - University of Education
4. Tran Thi Hong, TNU - University of Education
5. Nguyen Vu Bao, TNU - University of Education
6. Manivanh Yongsa, TNU - University of Education
7. Sy Danh Thuong, TNU - University of Education
8. Chu Hoang Mau, TNU - University of Education

Abstract

Hoya lockii is an endemic plant with many uses and medicinal properties. This is a rare species in Vietnam (the number of individuals in the wild is less than 50), listed in the Red Book for conservation at risk of extinction. Gene analysis, species identification, and the proposed use of barcodes are essential for preserving this valuable plant specimen. In this study, we used BLAST, BioEdit, and MEGA11 software to determine the gene region and build a phylogenetic tree of the studied species. The research team selected the rbcL and ndhF sequences with sizes of 1428 bp and 1519 bp, respectively, extracted from the chloroplast genome of H. lockii to compare with 17 species with high similarity in the NBCI. The analysis of the rbcL and ndhF gene regions indicated that the species of H.lockii is closely related to 4 species: H. hamiltoniorum, H. exilis, H. dimorpha, and H. megalaster. When using the rbcL marker, the reliability reached 80%, the ndhF marker gave a rate of 98%. With this result, the ndhF marker was proposed to be used as a barcode candidate for the identification of H. lockii species, and at the same time provided additional research data on a potential barcode for the phylogenetic analysis of plants.

Keywords

Hoya lockii; DNA Barcode; rbcL; ndhF; Species identification

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DOI: https://doi.org/10.34238/tnu-jst.12338

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