PREPARATION AND IN VITRO TEST OF INSECTICIDAL ACTIVITY OF NANO – BI AGAINST TETRANYCHUS SP.
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Received: 20/06/25                Revised: 14/11/25                Published: 18/11/25Abstract
This study focuses on the development, formulation, and in vitro evaluation of the biological efficacy of NANO-BIa novel nanoemulsion system composed of neem oil and cinnamon essential oildesigned for the effective control of Tetranychus sp. on tomato (Solanum lycopersicum) plants. Fourier-transform infrared spectroscopy analysis revealed characteristic alterations in functional groups, confirming the successful formation of the emulsion. The nanoformulation exhibited an average particle size of approximately 206 nanometers and a zeta potential of -55.7 mV, indicating excellent colloidal stability and dispersion uniformity. In bioefficacy trials, NANO-BI significantly outperformed the conventional chemical pesticide Vineem 1500EC, achieving an outstanding mortality rate of 98.33 - 100% within just 1 to 3 days at a concentration of 0.50%. Moreover, it maintained a high level of effectivenessranging from 95% to 100%even at the lowest tested concentration of 0.125%. These promising results suggest that NANO-BI is not only highly effective and fast-acting against Tetranychus sp., but also represents a strong candidate for sustainable, eco-friendly pest management solutions in agriculture.
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[1] J.‑C. Chen, Z.‑Z. Ma, Y.‑J. Gong, L.‑J. Cao, J.‑X. Wang, S.‑K. Guo, A. A. Hoffmann, and S.‑J. Wei, “Toxicity and control efficacy of an organosilicone to the two-spotted spider mite Tetranychus urticae and its crop hosts,” Insects, vol. 13, 2022, Art. no. 341, doi: 10.3390/insects13040341.
[2] S. Kumar, N. Singh, L. S. Devi, S. Kumar, M. Kamle, P. Kumar, and A. Mukherjee, “Neem oil and its nanoemulsion in sustainable food preservation and packaging: Current status and future prospects,” Journal of Agriculture and Food Research, vol. 7, 2022, Art. no. 100254, doi: 10.1016/j.jafr.2021.100254.
[3] T. T. Nguyen, T. H. Tran, D. Q. Le, T. H. Vu, D. H. Vu, N. T. Nguyen, T. T. H. Do, T. H. Nguyen, N. Q. Dang, and T. D. Tran, “Characterization and antifungal activity of limonoid constituents isolated from Meliaceae plants Melia dubia, Aphanamixis polystachya, and Swietenia macrophylla against plant pathogenic fungi in vitro,” Journal of Chemistry, 2021, Art. no. 4153790, doi: 10.1155/2021/4153790.
[4] N. Iqbal, D. K. Hazra, A. Purkait, A. Agrawal, and J. Kumar, “Bioengineering of neem nano-formulation with adjuvant for better adhesion over applied surface to give long term insect control,” Colloids and Surfaces B: Biointerfaces, vol. 209, 2022, Art. no. 112176.
[5] Y. Xie, Q. Huang, Z. Wang, H. Cao, and D. Zhang, “Structure–activity relationships of cinnamaldehyde and eugenol derivatives against plant pathogenic fungi,” Industrial Crops and Products, vol. 97, pp. 388–394, 2022.
[6] V. C. Bui, T. T. Le, T. H. Nguyen, N. T. Pham, H. D. Vu, X. C. Nguyen, Q. D. Tran, T. Hoang, Q. L. Dang, and T. D. Lam, “Curcumin-removed turmeric oleoresin nano-emulsion as a novel botanical fungicide to control anthracnose (Colletotrichum gloeosporioides) in litchi,” Green Processing and Synthesis, vol. 10, no. 1, pp. 729–741, 2021, doi: 10.1515/gps-2021-0071.
[7] Q. L. Dang, T. A. Nguyen, Q. D. Tran, T. T. T. Nguyen, X. M. Vu, C. Q. Nguyen, T. K. A. Vo, V. C. Bui, D. L. Tran, T. M. Tran, and T. K. Do, “Preparation and potential of nanoparticles containing curcuminoids to control fungal diseases in tropical fruits,” Vietnam Journal of Science and Technology, vol. 61, no. 2, pp. 242-252, 2023, doi: 10.15625/2525-2518/17620.
[8] D. Q. Pham, T. V. Chu, H. T. Phan, S. V. Nguyen, Q. D. Tran, H. K. Nguyen, A. T. K. Vo, D. L. Tran, K. N. Tran, H. D. Vu, and Q. D. Le, “Antifungal nanoformulation of botanical anthraquinone and TiO₂ against melon phytopathogenic fungi: preparation, in vitro bioassays and field test,” Notulae Botanicae Horti Agrobotanici Cluj-Napoca, vol. 53, 2025, Art. no. 14108, doi: 10.15835/nbha53114108.
[9] W. P. J. Overrmer, “Toxicological methods, spider mite,” in Spider Mites: Their Biology, Natural Enemies and Control, vol. 1(B), W. Helle and M. W. Sabelis, Eds. Amsterdam: Elsevier, 1985, pp. 183–189.
[10] A.-A. E. Ghada and Y. M. Naglaa, “Efficacy of cinnamon oil and its active ingredient (cinnamaldehyde) on the cotton mealy bug Hoccus solenopsis and the predator Chrysoperla carnea,” Bulletin of the National Research Centre, vol. 44, 2020, Art. no. 154, doi: 10.1186/s42269-020-00415-2.
[11] A. Plata-Rueda, J. M. Campos, G. S. Rolim, L. C. Martínez, and M. H. D. Santos, “Terpenoid constituents of cinnamon and clove essential oils cause toxic effects and behavior repellency response on granary weevil, Sitophilus granarius,” Ecotoxicology and Environmental Safety, vol. 156, pp. 263–270, 2018, doi: 10.1016/j.ecoenv.2018.03.016.
[12] P. Bueyong, M. J. Lee, S. K. Lee, S. B. Lee, I. H. Jeong, S. K. Park, Y. J. Jung, and H. S. Lee, “Insecticidal activity of coriander and cinnamon oils prepared by various methods against three species of agricultural pests (Myzus persicae, Tetranychus urticae, and Plutella xylostella),” Applied Biological Chemistry, vol. 60, no. 2, pp. 137–140, 2017, doi: 10.3839/jabc.2017.023.
[13] S.‑I. Kim, J.‑Y. Roh, D.‑H. Kim, H.‑S. Lee, and Y.‑J. Ahn, “Insecticidal activities of aromatic plant extracts and essential oils against Sitophilus oryzae and Callosobruchus chinensis,” Journal of Stored Products Research, vol. 39, pp. 293–303, 2003.
DOI: https://doi.org/10.34238/tnu-jst.13091
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