Email this article α-GLUCOSIDASE INHIBITORY ACTIVITY OF THREE GREEN SEAWEEDS COLLECTED IN THE COAST OF KHANH HOA PROVINCE
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Received: 01/03/20 Revised: 09/07/20 Published: 10/07/20Authors
1. Nguyen The Han
, Faculty of Food Technology, Nha Trang University2. Nguyen Thi Kim Hang, Le Hong Phong High School, Di Linh District, Lam Dong Province, Insitute of Biotechnology and Environment, Nha Trang University
3. Vu Le Quyen, Faculty of Food Technology, Nha Trang University
4. Ngo Thi Hoai Duong, Insitute of Biotechnology and Environment, Nha Trang University
Abstract
One of the effective therapeutic approaches in the management of type 2 diabetes is inhibition of α-glucosidase. This study investigated α-glucosidase inhibitory activity of three green seaweeds (Halimeda macroloba, Ulva reticulate and Ulva lactuca) and the results indicated that the inhibitory effect (IC50 values) of H. macroloba, U. reticulata and U. lactuca and were 3.98, 4.76 and 5.21 mg/ml, respectively. The suitable extraction time and extraction temperature for extracting high activity α-glucosidase inhibitors from green seaweed H. macroloba were found to be 60 min and 60 °C, respectively. Among the extract fractions, ethyl acetate showed the highest α-glucosidase inhibitory activity (IC50 value of 2.45 mg/ml), followed by n-hexane, butanol and water with IC50 values of 2.79, 4.11 and 4.91 mg/ml, respectively. Phytochemical screening showed phenolic, flavonoids and terpenoid were present in the ethyl acetate fraction of green seaweed H. macroloba.
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