APPLICATION OF SOME DNA BARCODE ANALYSIS SOFTWARE TO IDENTIFY MEDICINAL PLANTS
About this article
Received: 14/04/20                Revised: 08/06/20                Published: 11/06/20Abstract
The identification of medicinal plants with DNA barcodes is increasingly popular due to its high accuracy. To identify using DNA barcodes, researchers often use computer software to analyze and compare DNA barcodes of research samples with barcodes available on Genbank. Because the published gene sequence data increases rapidly in number, in order to conduct an effective analysis of these data, the software needs to be easy to use, able to calculate quickly, accurately and provide many statistical methods. In this group of software, three software programs, including BLAST software in NCBI, BioEdit v7.0.5.2, MEGA 7, are most commonly used due to meeting the above requirements. The article introduces three software programs to identify medicinal plants with illustrative examples of identifying the Jewels of Opar (Talinum paniculatum) pattern by ITS region barcodes. The research results of the paper show that the process of combining three software programs to identify medicinal plants by DNA gives the most accurate results.
Keywords
Full Text:
PDF (Tiếng Việt)References
[1]. H. Ledford, “Botanical identities: DNA barcoding for plants comes a step closer,” Nature, vol. 451, pp. 451-616, 2008.
[2]. S. Chen, H. Yao, J. Han, C. Liu, J. Song, L. Shi, Y. Zhu, X. Ma, T. Gao, X. Pang, K. Luo, Y. Li, X. Li, X. Jia, Y. Lin, and C. Leon, “Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species,” PLoS One, vol. 5, no. 1, p. e8613, 2010.
[3]. Y. Zuo, Z. Chen, K. Kondo, T. Funamoto, J. Wen, and S. Zhou, “DNA barcoding of Panax species,” Planta Med, vol. 77, no. 2, pp. 182-187, 2011.
[4]. T. H. Le, N. L. Nguyen, M. M. Bui, H. H. Ha, T. T. H. Huynh, V. H. Nong, V. H. Ha, and T. T. H. Le, “Application of DNA barcodes in identification of ginseng samples in the genus Panax L.,” Viet Nam Journal of Biotechnology, vol. 15, no. 1, pp. 63-72, 2017.
[5]. T. D. Nguyen, Q. N. Nguyen, N. L. Tran, T. T. Nguyen, T. P. Ninh, T. T. N. Doan, T. T. H. Le, and N. L. Nguyen, “Morphological characteristics and DNA barcodes of one-leafed seven-flowered tree, Paris vietnamensis (Takht.) H.li, in Vietnam,” Vietnam Journal of Agricultural Sciences- VJAS, vol. 16, no. 4, pp. 282-289, 2017.
[6]. T. N. T. Vu, M. T. Ho, V. S. Le, T. T. Nguyen, and H. M. Chu, “Morphological characteristics of Talinum paniculatum, and nucleotide sequences of ITS region, rpoC1 and rpoB genes,” Viet Nam Journal of Biotechnology, vol. 16, no. 3, pp. 451- 458, 2018.
[7]. S. F. Altschul, W. Gish, W. Miller, E. W. Myers, and D. J. Lipman, “Basic local alignment search tool,” J. Mol Biol, vol. 215, no. 3, pp. 403-410, 1990.
[8]. Wikipedia, “BLAST,” 2020. [Online]. Available: https://vi.wikipedia.org/wiki/BLAST [Accessed May 30, 2020].
[9]. Biological Journal, “Compare sequences - BLAST online”, 2020. [Online]. Available: https://tapchisinhhoc.com/so-sanh-trinh-tu-blast-online.html/. [Accessed April 09, 2020].
[10]. T. A. Hall, “BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT,” Nucl. Acids. Symp. Ser., vol. 41, pp. 95-98, 1999.
[11]. S. Kumar, K. Tamura, and M. Nei, “MEGA: Molecular Evolutionary Genetics Analysis software for microcomputers,” Comput Appl Biosci., vol. 10, no. 2, pp. 189-191, 1994.
[12]. Biology of Vietnam, “Build phylogenetic tree easily with BLAST-MEGA”, 2020. [Online]. Available: https://sinhhocvietnam.com/xay-dung-cay-phat-sinh-loai-de-dang-voi-blast-MEGA/. [Accessed April 09, 2020]
[13]. S. Kumar, G. Stecher, and K. Tasuma, “MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets,” Mol Biol Evol, vol. 33, pp. 1870 – 1874, 2016.
Refbacks
- There are currently no refbacks.





