Email this article RESEARCH ON PROCEDURE THE MICROPROPAGATION FOR ORCHID SPECIES KIEM “PHAN TRI” (Cymbidium finlaysonianum Lindl.)
About this article
Received: 16/06/20 Revised: 28/07/20 Published: 31/07/20Authors
1. Nguyen Thi Diep
, Research & Development Center For High Technology Agricuture2. Huynh Thi Kim, Research & Development Center For High Technology Agricuture
Abstract
The orchid species Kiem Phan Tri (belonging to genus Cymbidium) is a species of orchid that grows very hard and long leaves, mutant yellow flowers that have been famous for a long time in Vietnam. In the study of propagating the orchid species Kiem Phan Tri, young shoots with length of 20 - 30 cm were used to the starting culture material. When dormant buds reached 2 cm, their tops were cut and used as experiment materials. The results showed that on MS medium containing 3 mg/l BA and 0.5 mg/l NAA, the precentage of PLBs regeneration from the top of buds was 64.44% and 17.2 PLBs/explant. The type of culture using the RITA® temporary was submerged culture system, with the increase in the PLBs weight and the number of regenerated shoots was 18.7 times and 84.6 shoots respectively. Hyponex® medium with N:P:K consist of 20:20:20 combining with 15 g/L bananas for a rooting rate of 100% showed the shoots height are 6.3 cm with 3.67 roots. Survival rate of plantlets in nursery garden was 95%.
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