ISOLATION AND SELECTION OF BACILLUS STRAINS WITH CAPABILITY IN PRODUCTION OF POLYGLUTAMIC ACID
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Received: 17/07/20                Revised: 30/07/20                Published: 31/07/20Abstract
Curently, attentions are drawn to studies about application of active substances produced by microbes in environmental treatment. Polyglutamic acid (PGA) is one of biological polymers that has multiple applications in different fields and has a great potential use as biological coagulant in water treatment. Bacteria under Bacillus group are useful in production of biologically active substances and have a significant role in PGA production. This study aimed to isolate and select Bacillus bacteria from fermented soya been product in order to provide seed strain for further investigations on production and application of PGA in the role of biological coagulant. After using isolation, selection and verification methods by FT-IR infrared spectroscopy, four Bacillus strains from Natto fermented soybean products were obtained, of which two strains have been selected with better PGA production performance B11, B33, is a prerequisite for PGA production for further studies.
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[1]. M. Marvasi, P. T. Visscher, and L. C. Martinez, “Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis,” FEMS microbiology letters, vol. 313(1), pp. 1-9, 2010.
[2]. I. N. Najar, and S. Das, “Poly-glutamic acid (PGA)-Structure, synthesis, genomic organization and its application: A Review,” International Journal of Pharmaceutical Sciences and Research, vol. 6(6), pp. 2258-2280, 2015.
[3]. I. B. Bajaj, and R. S. Singhal, "Sequential Optimization Approach for Enhanced Production of Poly-γ-Glutamic Acid from Newly Isolated Bacillus subtilis," Food Technology and Biotechnology, vol. 47(3), pp. 313–322, 2009.
[4]. E. Baruch, A. M. Belostotskii, and Y. Mastai, "Relationship between the antifreeze activities and the chemical structures of polyols," Journal of Molecular Structure, vol. 874(1–3), pp. 170-177, 2008.
[5]. V. Campos, J. M. Domingos, D. N. Anjos, and V. S. Lira, “Study of fluvial water treatability using γ-polyglutamic acid based biopolymer coagulant,” Anais da Academia Brasileira de Ciencias, vol. 91(3). pp. 1-8, 2019.
[6]. B. Manocha, and A. Margaritis, "A novel Method for the selective recovery and purification of γ‐polyglutamic acid from Bacillus licheniformis fermentation broth," Biotechnology progress, vol. 26(3), pp. 734-742, 2010.
[7]. M. Ashiuchi, T. Kamei, D. H. Baek, S. Y. Shin, M. H. Sung, K. Soda, T. Yagi, and H. Misono, "Isolation of Bacillus subtilis (chungkookjang), a poly-γ-
glutamate producer with high genetic competence," Applied Microbiology and Biotechnology, vol. 57(5-6), pp. 764-769, 2001.
[8]. I. N. Bajaj, and R. S. Singhal, "Flocculation Properties of Poly γ Glutamic Acid Produced from Bacillus subtilis Isolate," Food and Bioprocess Technology, vol. 4(5), pp. 745-752, 2011.
[9]. J. H. Zhang, and M. Luo, "Cloning and Expression of the γ -Polyglutamic Acid Synthetase Gene pgs BCA in Bacillus subtilis WB600," BioMed Research International, vol. 2016, vol. 1-7, 2016.
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