RESEARCH ON THE HYDROLYSIS PROCESS OF SOYBEAN POWDER BY ALCALASE TO PRODUCE COMMERCIAL FERTILIZER

Abstract

The aim of the study was to investigate the single factor of the appropriate mixture ratio between enzyme and EM, the ratio of supplemental irrigation, incubation temperature, and hydrolysis time affecting the hydrolysis process of soybean residues with alcalase enzyme. The dissolver protein content in hydrolysates is determined by the Lowry method. The corresponding survey results were EM 2% + E 1.5%; 4 (ml/g); 45 ºC; 8 hours, respectively. On the basis of investigating the factors affecting the hydrolysis conditions, we found that the mixture ratio of enzymes and EM, the ratio of supplemental irrigation, incubation temperature, and hydrolysis time were strongly influencing factors to the hydrolysis process. By employing Box- Behnken design, the optimal condition for the hydrolysis process of soybean residues was found, of which the mixture ratio of enzyme and EM was EM 2% + E 1.5%, the ratio of supplemental irrigation: 4.2, incubation temperature 45ºC, hydrolysis time 8.53 hours. The experimental result showed a high compatibility with the model.