SELECTION AND DETERMINATION OF BIOLOGICAL CHARACTERISTICS BACTERIA STRAINS CAPABLE OF PRODUCING INDOLE-3-ACETIC ACID (IAA)

Đỗ Tất Thịnh; Lê Xuân Sơn; Trần Thị Nhàn; Nguyễn Thị Kim Oanh; Nguyễn Văn Hiếu

doi:10.34238/tnu-jst.8098

SELECTION AND DETERMINATION OF BIOLOGICAL CHARACTERISTICS BACTERIA STRAINS CAPABLE OF PRODUCING INDOLE-3-ACETIC ACID (IAA)

About this article

Received: 09/06/23 Revised: 04/08/23 Published: 04/08/23

Authors

1. Do Tat Thinh , Institute of Tropical Ecology - Vietnam-Russian Tropical Centre
2. Le Xuan Son, Institute of Tropical Ecology - Vietnam-Russian Tropical Centre
3. Tran Thi Nhan, Institute of Tropical Ecology - Vietnam-Russian Tropical Centre
4. Nguyen Thi Kim Oanh, Institute of Tropical Ecology - Vietnam-Russian Tropical Centre
5. Nguyen Van Hieu, Institute of Biotechnology - VAST

Abstract

Indole-3-acetic acids (IAA) are phytohormones belonging to the auxins. IAA is produced by groups of microorganisms living in the rhizosphere, such as Pseudomonas, Azotobacter, Enterobacter, Alcaligenes, Bacillus, and Serratia. Among 132 bacterial strains isolated from soil samples collected at nature reserves of Bat Xat, Kon Chu Rang and national parks Phia Oac - Phia Den of Vietnam capable of producing IAA, 14 strains are capable of producing IAA >20 ug/ml. A study on the biological characteristics of those two strains showed that they could grow well on carbon sources such as D-glucose, D-mannitol, D-fructose, D-raffinose and Saccharose, and nitrogen sources such as L-tryptophan, L-tyrosine, meat extract, peptone and NH₄NO₃. Favorable temperature and pH for their growth were established, i.e. temperatures of 15-37°C, and pH of 5 to 9. All two strains can produce extracellular enzymes such as amylase, cellulase and protease. Classification based on 16S rRNA gene sequences revealed high similarity (>99%) between our strains and the GenBank reference, i.e. strain PPM4.11(OQ134060) is homologous to Enterobacter cloacae ATCC13047(NR118568) and strain PPM4.5 (OQ149978) is homologous to Priestia megaterium strain NBRC15308 (NR112636). The PPM4.5 and PPM4.11 strains increased the mean germination of green mustard seeds by 10% and 10.67% and water spinach seeds by 7.67% and 5.33%.

Keywords

Indole-3-acetic acid (IAA); Auxin; Bacillus; Biological characteristics; Root zone bacteria

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DOI: https://doi.org/10.34238/tnu-jst.8098

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