DEVELOPMENT AND VALIDATION OF A HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ANALYTICAL METHOD FOR THE DETERMINATION α-MANGOSTIN IN MANGOSTEEN PEEL

Huỳnh Thị Như Quỳnh; Trần Lưu Phúc; Hà Hoàng Anh Vĩnh; Hoàng Thị Thu Huyền; Lê Trung Khoảng; Huỳnh Văn Chung

doi:10.34238/tnu-jst.8182

DEVELOPMENT AND VALIDATION OF A HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ANALYTICAL METHOD FOR THE DETERMINATION α-MANGOSTIN IN MANGOSTEEN PEEL

About this article

Received: 21/06/23 Revised: 15/08/23 Published: 18/08/23

Authors

1. Huynh Thi Nhu Quynh, Buon Ma Thuot Medical University
2. Tran Luu Phuc, Buon Ma Thuot Medical University
3. Ha Hoang Anh Vinh, Buon Ma Thuot Medical University
4. Hoang Thi Thu Huyen, Buon Ma Thuot Medical University
5. Le Trung Khoang, Buon Ma Thuot Medical University
6. Huynh Van Chung , Buon Ma Thuot Medical University

Abstract

In this study, a procedure for quantifying α-mangostin in mangosteen peel by high performance liquid chromatography (HPLC) with a PDA detector was carried out. Chromatographic separation was achieved using a symmetry C18 column (4.6 mm x 250 mm) with the mobile phase consisting of acetonitril : water (95 : 5, v/v) at a flow rate of 1.5 ml/min, and monitored at 320 nm. The method was validated in accordance with International Conference on Harmonisation (ICH) guidelines. The calibration curve was linear over the concentration range from 0.01 to 200 µg/ml. The limit of detection (LOD) was 0.01 µg/ml and the limit of quantitation (LOQ) was 0.03 µg/ml. The method achieved accuracy with recovery in the range of 99.2 – 101.9%, high repeatability with an RSD of 0.746 % for peak area and 0.074 % for retention time. The procedure was applied to determine the α-mangostin content in mangosteen peels of several different regions in Viet Nam.

Keywords

α-mangostin; Mangosteen; HPLC; Validation; Determination

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DOI: https://doi.org/10.34238/tnu-jst.8182

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