SELECTION OF EFFECTIVE Bacillus STRAINS TO CONTROL BACTERIAL LEAF BLIGHT OF RICE CAUSED BY THE Xanthomonas oryzae pv. oryzae ISOLATED FROM SOME RICE GROWING AREAS IN THE MEKONG DELTA

Hồ Xuân Uyển; Trần Vũ Phến; Phùng Ngọc Yến Phương

doi:10.34238/tnu-jst.8691

SELECTION OF EFFECTIVE Bacillus STRAINS TO CONTROL BACTERIAL LEAF BLIGHT OF RICE CAUSED BY THE Xanthomonas oryzae pv. oryzae ISOLATED FROM SOME RICE GROWING AREAS IN THE MEKONG DELTA

About this article

Received: 06/09/23 Revised: 17/10/23 Published: 18/10/23

Authors

1. Ho Xuan Uyen , College of Agriculture – Can Tho University
2. Tran Vu Phen, College of Agriculture – Can Tho University
3. Phung Ngoc Yen Phuong, College of Agriculture – Can Tho University

Abstract

This research investigated the ability to control bacterial leaf blight (BLB) of rice caused by three strains of Xanthomonas oryzae pv. oryzae (Xoo) of 10 strains of Bacillus spp. based on its in vitro antagonistic ability to Xoo, and its effectiveness in BLB disease suppression under greenhouse conditions. Each experiment consisted of 2 factors, completely randomized design, with 4 replications. The results showed that 3 strains of Bacillus, i.e. BR15, B42 and B61 showed very strong antagonistic activity against all three strains of Xoo, with the inhibition zone radius at 7 days of 17.93 mm, 14.93 and 14.53 mm, respectively. Under net house conditions, all three strains B42, B61 and BR15 showed high BLB disease suppression, in which, strain BR15 had superior (48.94%), with higher efficiency than the control (Starner 20WP). Consequently, it can be seen that BR15 is a promising Bacillus strain and can be used for further study in the production of biological products.

Keywords

Bacillus; Bacterial leaf blight; Xanthomonas oryzae pv. oryzae; Control; Rice

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References

[1] S. S. Gnanamanickam, Biological Control of Rice Diseases. Springer, 2009.

[2] Y. S. Ooi, N. M. I. Mohamed Nor, G. Furusawa, M. Tharek, and A. H. Ghazali, “Application of Bacterial Endophytes to Control Bacterial Leaf Blight Disease and Promote Rice Growth,” The Plant Pathology Journal, vol. 38, no. 5, pp. 490-502, Oct. 2022, doi: 10.5423/ppj.oa.01.2022.0014.

[3] M. M. Rahman et al., “Potential Role of Rice Plant Growth Promoting Phylloplane and Rhizospheric Bacteria in Controlling Xanthomonas oryzae pv. oryzae,” in Integrative advances in rice research. M. Huang, Ed. London: Intechopen, 2022.

[4] X. Chen et al., “Melatonin Treatment Inhibits the Growth of Xanthomonas oryzae pv. oryzae,” Frontiers in Microbiology, vol. 9, Oct. 2018, doi: 10.3389/fmicb.2018.02280.

[5] S.-I. Kim, J. T. Song, J.-Y. Jeong, and H. S. Seo, “Niclosamide inhibits leaf blight caused by Xanthomonas oryzae in rice,” Scientific Reports, vol. 6, no. 1, Feb. 2016, doi: 10.1038/srep21209.

[6] X. Q. Wang, D. L. Zhao, L. L. Shen, C. L. Jing, and C. S. Zhang, “Application and Mechanisms of Bacillus subtilis in Biological Control of Plant Disease,” Role of Rhizospheric Microbes in Soil, pp. 225-250, 2018, doi: 10.1007/978-981-10-8402-7_9.

[7] F. U. Rajer et al., “Bacillus spp.-Mediated Growth Promotion of Rice Seedlings and Suppression of Bacterial Blight Disease under Greenhouse Conditions,” Pathogens, vol. 11, no. 11, p. 1251, Oct. 2022, doi: 10.3390/pathogens11111251.

[8] R. Lahlali et al., “Biological Control of Plant Pathogens: A Global Perspective,” Microorganisms, vol. 10, no. 3, p. 596, Mar. 2022, doi: 10.3390/microorganisms10030596.

[9] F. Lemessa and W. Zeller, “Screening rhizobacteria for biological control of Ralstonia solanacearum in Ethiopia,” Biological Control, vol. 42, no. 3, pp. 336-344, Sep. 2007, doi: 10.1016/j.biocontrol.2007.05.014.

[10] N. A. Ahmed và E. B. Zahran, “Inhibition of soil borne Xanthomonas campestris pv. malvacearum in cotton by Bacillus spp.,” in Proceedings of the 1st International Symposium on Biological Control of Bacterial Plant Diseases, W. Zeller và C. Ullrich, Eds., Berlin, Germany: Biologische Bundesanstalt für Land- und Forstwirtschaft, 2006, pp. 86-92.

[11] T. W. Mew, “Scanning Electron Microscopy of Virulent and Avirulent Strains of Xanthomonas campestrispv. oryzaeon Rice Leaves,” Phytopathology, vol. 74, no. 6, p. 635, 1984, doi: 10.1094/phyto-74-635.

[12] S. S. Gnanamanickam, V. B. Priyadarisini, N. N. Narayanan, P. Vasudevan, and S. Kavitha, “An overview of bacterial blight disease of rice and strategies for its management,” Current Science, vol. 77, no. 11, pp. 1435-1444, 1999.

[13] C. Kannan, D. Mishra, G. Rekha, P. Maruthi, H. Shaik, and R. M. Sundaram, “Diversity analysis of antagonistic microbes against bacterial leaf and fungal sheath blight diseases of rice,” Egyptian Journal of Biological Pest Control, vol. 31, no. 1, Aug. 2021, doi: 10.1186/s41938-021-00462-x.

DOI: https://doi.org/10.34238/tnu-jst.8691

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