DISEASE REDUCING EFFECTS AND ENZYME ACTIVITIES RELATED TO ANTHRACNOSE RESISTANCE MECHANISM OF CUCUMBER CAUSED BY Colletotrichum lagenarium OF Bacillus sp. MIXTURE UNDER NET HOUSE CONDITIONS | Huy | TNU Journal of Science and Technology

DISEASE REDUCING EFFECTS AND ENZYME ACTIVITIES RELATED TO ANTHRACNOSE RESISTANCE MECHANISM OF CUCUMBER CAUSED BY Colletotrichum lagenarium OF Bacillus sp. MIXTURE UNDER NET HOUSE CONDITIONS

About this article

Received: 08/09/23                Revised: 14/11/23                Published: 15/11/23

Authors

1. Le Hieu Huy, Institute of Food and Biotechnology, Can Tho University
2. Nguyen Thi Lien Email to author, Institute of Food and Biotechnology, Can Tho University
3. Nguyen Dac Khoa, Institute of Food and Biotechnology, Can Tho University

Abstract


Anthracnose cucumber caused by the fungus Colletotrichum lagenarium seriously affected the yield and quality of cucumber. This study aims at testing for the disease-reducing effects, catalase (CAT), and phenylalanine ammonia-lyase (PAL) enzyme activities related to the mechanism of disease resistance stimulation by seed treatment with two strains of bacteria Bacillus amyloliquefaciens VL4.6 and Bacillus siamensis CL8. The bacterial mixing ratio was investigated in five ratios: 1:0, 1:1, 1:2, 2:1, and 0:1, with a microbial density of 108 cells/mL. The percentage of infected leaf area was recorded every 24 hours for seven days after inoculation. The ratio of bacteria 1:1 was the most effective in disease reduction with the ratio of diseased leaf area of 7.5% (bacteria – with inoculation) equivalent to that of the positive control 6.8% (CaCl2 – with inoculation), and lower than that of negative control 30.7% (water – with inoculation) at seven days after inoculation. CAT and PAL activities in leaf tissue were increased when treated with Bacillus mixture, especially in the case of pathogens attacking the plant. CAT and PAL activities peaked in leaf tissue four days after inoculation. The results showed that mixing B. amyloliquefaciens VL4.6 and B. siamensis CL8 at a ratio of 1:1 (density 108 cells/mL) was the best treatment for disease reduction.

Keywords


Bacillus amyloliquefaciens; Bacillus siamensis; Anthracnose; Colletotrichum lagenarium; Resistance

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DOI: https://doi.org/10.34238/tnu-jst.8709

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