BIOLOGICAL CHARACTERISTICS OF ENTOMOPATHOGENIC FUNGUS CORDYCEPS TAKAOMONTANA FROM HOANG LIEN AND PU MAT NATIONAL PARKS

Nguyễn Thị Thanh Lan; Đinh Thị Ngọc Thuý; Nguyễn Trần Trung; Đỗ Thị Tuyến; Ngô Cao Cường; Phí Quyết Tiến; Nguyễn Thị Diệu Thuý

doi:10.34238/tnu-jst.8780

BIOLOGICAL CHARACTERISTICS OF ENTOMOPATHOGENIC FUNGUS CORDYCEPS TAKAOMONTANA FROM HOANG LIEN AND PU MAT NATIONAL PARKS

About this article

Received: 20/09/23 Revised: 24/10/23 Published: 24/10/23

Authors

1. Nguyen Thi Thanh Lan , Institute of Biotechnology – VAST; Graduate University of Science and Technology - VAST
2. Dinh Thi Ngoc Thuy, Institute of Biotechnology – VAST
3. Nguyen Tran Trung, Institute of Biotechnology – VAST
4. Do Thi Tuyen, Vietnam - Russia Tropical Science and Technology Research Center; Graduate University of Science and Technology - VAST
5. Ngo Cao Cuong, Vietnam - Russia Tropical Science and Technology Research Center; Graduate University of Science and Technology - VAST
6. Phi Quyet Tien, Institute of Biotechnology – VAST; Graduate University of Science and Technology - VAST
7. Nguyen Thi Dieu Thuy, Institute of Biotechnology – VAST; Graduate University of Science and Technology - VAST

Abstract

A total of 9 isolates of insect parasitic fungi were isolated from Hoang Lien and Pu Mat National Parks. On potato dextrose agar medium (PDA), the mycelium of all isolates is white, flourish, and porous. The mycelium can intertwine to form fruit bodies on a porous fermentation medium containing brown rice and silkworm pupae. The ITS gene sequence analysis results and construction of a genetic taxonomic tree based on sequence homology with reference strains showed that 9 analyzed isolates belonged to Cordyceps takaomontana species. By analyzing liquid chromatography-mass spectrometry (LC/MS), it was determined that 7/9 isolates contain at least 1 active ingredient adenosine, beauvericin, or mannitol, and 3/9 isolates are active with all 3 active ingredients. VN6 isolate was selected for research because of its ability to grow and develop strongly. VN6 isolates produced fruiting bodies after 12 days on porous fermentation medium at temperatures 25°C and moisture 80%, the content of active ingredients adenosine, beauvericin, and mannitol reached 0,85; 2,92 and 5,57 mg/g, respectively. Obtained results reveal that the isolate C. takaomontana VN6 was evaluated as having the potential to be exploited as a medicinal plant and selected for further applied research.

Keywords

Cordyceps takaomontana; Pharmacy material; Insect parasitic mushroom; Hoang Lien National Park; Pu Mat National Park

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DOI: https://doi.org/10.34238/tnu-jst.8780

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