EVALUATION OF THE ANTICANCER ACTIVITY OF POUZOLZIA PENTANDRA EXTRACTS AND ISOLATED COMPOUNDS

Nguyễn Anh Hưng; Nguyễn Quang Hợp; Hà Thị Minh Tâm; Vũ Thị Giang; Đỗ Tiến Lâm; Nguyễn Thị Huệ; Vũ Thành Đạt; Nguyễn Ngọc Linh; Vũ Thị Thu Lê

doi:10.34238/tnu-jst.8782

EVALUATION OF THE ANTICANCER ACTIVITY OF POUZOLZIA PENTANDRA EXTRACTS AND ISOLATED COMPOUNDS

About this article

Received: 20/09/23 Revised: 30/09/23 Published: 16/10/23

Authors

1. Nguyen Anh Hung , Hanoi Pedagogical University 2
2. Nguyen Quang Hop, Hanoi Pedagogical University 2
3. Ha Thi Minh Tam, Hanoi Pedagogical University 2
4. Vu Thi Giang, Graduate University of Science and Technology - VAST
5. Do Tien Lam, Graduate University of Science and Technology - VAST; Institute of Natural Products Chemistry – VAST
6. Nguyen Thi Hue, Institute of Natural Products Chemistry – VAST
7. Vu Thanh Dat, Institute of Natural Products Chemistry – VAST
8. Nguyen Ngoc Linh, Thanh Do University
9. Vu Thi Thu Le, TNU - University of Agriculture and Forestry

Abstract

From the extraction of n-hexane fraction from the Pouzolzia pentandra plant using chromatographic methods, combined with recrystallization in suitable solvents, compounds were isolated: β-sitosterol (1), β-sitosterol-3-O-β-D-glucopyranoside (5), (20S)-20-hydroxydammar-24-en-3-one (4), quercetin (3), and kaempferol (2). Their chemical structures were determined through one- and two-dimensional nuclear magnetic resonance (NMR) spectroscopy. The remaining fractions were further isolated and their chemical structures were determined. The results of biological activity tests on cancer cell lines HepG2, PC3, and A539 indicated that the extracts and pure compounds exhibited activity at different levels. Among them, compound 4 showed the best cytotoxic activity against liver cancer cell line (HEP-G2) with IC₅₀ value of 14.65 µg/mL. This research has supplemented and enriched the chemical composition and biological activities of plants in the genus Pouzolzia in general and the species Pouzolzia pentandra in particular.

Keywords

Pouzolzia pentandra; HepG2; PC3; A539; Kaempferol; Quercetin

Full Text:

PDF (Tiếng Việt)

References

[1] X.-M. Chen and Z.-H. Li, “Effect of FPZ, a total flavonoids ointment topical application from Pouzolzia zeylanica var. microphylla, on mice skin infections”, Brazilian Journal of Pharmacology, vol. 28, no. 3, pp. 732-737, 2018.

[2] M. Q. Ahsan, M. T. Alam, M. M. U. Chowdhury, M. T. Nasim, and S. M. S. Islam, “In Vitro and In Vivo Evaluation of Pharmacological Activites of Pouzolzia sanguinea,” Journal of Bio-Science, vol. 29, no. 2, 2021, doi: 10.3329/jbs.v29i2.54952.

[3] V. C. Vo, Dictionary of Vietnamese medicinal plants I. Medical Publishing House, pp. 189-192, 2011.

[4] A. Ghafoor, Urticaceae, Flora of Pakistan, 1981, p.137.

[5] S. Abu, M. A. Sattar, et al., “A prenylated isoflavone from Pouzolzia zeylanica: its in vitro antimicrobial activity and cytotoxic evaluation,” Oriental Journal of Chemistry, vol. 19, no. 1, pp. 35-40, 2003.

[6] F. Ming and Y. Y. Niu, “Study on the chemical constituents in Pouzolzia zeylanica, Zhongyaoca,” Journal of Chinese medicinal materials, vol. 35, no. 11, pp. 1778-1781, 2012.

[7] C. Sangsuwon, W. Jiratchariyakul, Y. U-pratya, and T. Kummalue, “Antiproliferative Effect and the Isolated Compounds of Pouzolzia indica,” Evidence-Based Complementary and Alternative Medicine, vol. 2013, pp. 1-8, 2013, doi: 10.1155/2013/342352.

[8] W. Lujun and X. Zhelong, “Chemical Constituents of Pouzolzia zeylanica with PPARγ and PPARβ Activities,” Chemistry of Natural Compounds, vol. 51, no. 6, pp. 1157-1159, 2015.

[9] S. Brazendranath and R. S. M. Abu, “Phytochemical and Biological Activity Studies on Pouzolzia zeylanica (Linn.) Benn,” National University Journal of Science, vol. 1, no. 1, pp. 51-58, 2014.

[10] I. S. Sarma and B. Dinda, “A new friedelane triterpene ester from Pouzolzia indica,” Indian Journal of Chemistry, vol. 52B, 2013, doi: 10.1002/CHIN.201420193.

[11] Z.-H. Chen, H. Zhang, S.-H. Tao, Z. Luo, and C.-Q. Zhong, “Norlignans from Pouzolzia zeylanica var. microphylla and their nitric oxide inhibitory activity,” Journal of Asian Natural Products Research, vol. 17, no. 10, pp. 959-966, 2015.

[12] T. H. N. Le, T. Q. Phan, T. T. Trinh, T. T. Nguyen, T. H. A. Nguyen, T. T. X. Do, X. N. Nguyen, H. T. Bui, and V. K. Phan, “New Neolignan and Dihydrostilbene Derivatives from Pouzolzia sanguinea Inhibit NO Production in LPS-Activated BV2 Cells,” Natural Product Communications, vol. 15, no. 8, pp. 1-6, 2020.

[13] M. Mohammed, A. R. Maxwell, R. Ramsewak, and W. F. Reynolds, “Norlignans from Pouzolzia occidentalis,” Phytochemistry Letters, vol. 3, no. 1, pp. 29-32, 2010.

[14] T. H. N. Le, T. M. H. Pham, T. A. Nguyen, H. T. Bui, X. N. Nguyen, V. D. Vu, H. H. Nguyen, Y. Seo, S. H. Kim, and V. K. Phan, “Two new norlignans from the aerial parts of Pouzolzia sanguinea (Blume) Merr,” Natural Product Research, vol. 5, pp. 1-8, 2020.

[15] T. T. Le, "Chemical composition of the fish sauce plant (Pouzolzia zeylanica)," Master's thesis report in chemistry, Ho Chi Minh University of Natural Sciences, 2007.

[16] V. A. Nyigo, X. Peter, F. Mabiki, H. M. Malebo, R. H. Mdegela, and G. Fouche, “Isolation and identification of euphol and β-sitosterol from the dichloromethane extracts of Synadenium glaucescens,” The Journal of Phytopharmacology, vol. 5, no. 3, pp. 100-104, 2016.

[17] S. Mouffok, H. Haba, C. Lavaud, C. Long, and M. Benkhaled, “Chemical constituents of Centaurea omphalotricha Coss. & Durieu ex Batt. & Trab,” Records of Natural Products, vol. 6, 2018, Art. no. 292.

[18] I. E. Smirnovaa, H. Do Thi Thub, O. B. Kazakovaa, G. A. Tolstikova, O. S. Kukovinetsa, A. N. Lobova, and K. Yu, “SuponitskiiOzonolysis of Dipterocarpol and Its Derivatives”. Russian Journal of Organic Chemistry, vol. 48, no. 10, pp. 1370-1376, 2012.

DOI: https://doi.org/10.34238/tnu-jst.8782

Refbacks

There are currently no refbacks.



Remember me