CHEMICAL COMPOSITION AND IN VITRO ANTI-INFLAMMATORY ACTIVITY OF POUZOLZIA ZEYLANICA IN VIETNAM

Nguyễn Anh Hưng; Nguyễn Quang Hợp; Hà Thị Minh Tâm; Quản Cẩm Thúy; Quách Văn Linh; Nguyễn Ngọc Linh; Vũ Thị Thu Lê

doi:10.34238/tnu-jst.8806

CHEMICAL COMPOSITION AND IN VITRO ANTI-INFLAMMATORY ACTIVITY OF POUZOLZIA ZEYLANICA IN VIETNAM

About this article

Received: 23/09/23 Revised: 24/10/23 Published: 25/10/23

Authors

1. Nguyen Anh Hung , Hanoi Pedagogical University 2
2. Nguyen Quang Hop, Hanoi Pedagogical University 2
3. Ha Thi Minh Tam, Hanoi Pedagogical University 2
4. Quan Cam Thuy, Viet Tri University of Industry
5. Quach Van Linh, Viet Tri University of Industry; Centre for Diesease control of Bac giang province
6. Nguyen Ngoc Linh, Thanh Do University
7. Vu Thi Thu Le, TNU - University of Agriculture and Forestry

Abstract

The Pouzolzia zeylanica commonly found in tropical regions of South Asia, Southeast Asia, and other warm regions around the world. The main chemical components belong to the triterpenoid, flavonoids and lignans, which proven to have extremely diverse biological activities such as anti-inflammatory, strengthening blood vessel walls, anti-toxic, reducing liver damage, anti-oxidation, anti-virus and cancer. From the stem and leaves of Pouzolzia zeylanica, three compounds have been isolated and determined: Naringenin (1), bauerenol (2) and vitexin (3). The results of testing the in vitro anti-inflammatory activity of the fractions and isolated compounds obtained from Pouzolzia zeylanica was evaluated through the inhibition of LPS-triggered NO production on RAW 264.7 macrophages, the results showed total residue (PZ) at a concentration of 100 µg/ml has the ability to strongly inhibit NO production and is not toxic to cells with an IC₅₀ of 15.24 µg/ml. The n-hexane (PZH) fraction exhibited good anti-inflammatory activity with an IC₅₀ of 12.08 µg/ml. Compound vitexin (3) has the ability to strongly inhibit NO production and is not toxic to cells with an IC₅₀ of 20.85 µM. The above results guide further research on chemical composition and anti-inflammatory activity in the future.

Keywords

Pouzolzia zeylanica; Vitexin; Naringenin; Bauerenol; Anti-inflammatory

Full Text:

PDF (Tiếng Việt)

References

[1] H. P. Kim, K. H. Son, H. W. Chang, and S. S. Kang, “Anti-inflammatory plant flavonoids and cellular action mechanisms,” Journal of Pharmacological Sciences, vol. 96, pp. 229-245, 2004.

[2] A. R. Amin, M. Attur, and S. B. Abramson, “Nitric oxide synthase and cyclooxygenases: distribution, regulation, and intervention in arthritis,” Curr Opin Rheumatol, vol. 11, pp. 202-209, 1999.

[3] J. N. Sharma, A. Al Omran, and S. S. Parvathy, “Role of nitric oxide in inflammatory diseases,” Inflammopharmacology, vol. 15, pp. 252-259, 2007.

[4] D. M. La, M. H. Tran, D. H. Duong, H. T. Tran, and K. B. Ninh, Vietnam plant resources - Plants containing biologically active compounds I. Agriculture Publishing House, 2009, pp. 2-15.

[5] T. C. Le, Some basic characteristics of Vietnam's flora. Science and Technology Publishing House, 1999, p. 307.

[6] H. H. Pham, Vietnamese plants II. Ho Chi Minh City Youth Publishing House, 2000, pp. 602-603.

[7] V. C. Vo, Dictionary of Vietnamese medicinal plants I. Medical Publishing House, 2011, pp. 189-192.

[8] D. Saha et al., “Antifungal Activity of ethanol extract of Pouzolzia zeylanica (L.) Benn,” International Journal of Pharmaceutical Teaching & Practices, vol. 3, no. 1, p. 272, 2012.

[9] C. Yanfen, L. I. Kaiying et al., “Therapeutic Effect and Mechanism of Pouzolzia zeylanica on Skin Ulcers in Rats [J],” Traditional Chinese Drug Research and Clinical Pharmacology, vol. 3, 2013, Art. no. 011.

[10] X.-M. Chen, Z.-H. Li et al., “Effect of FPZ, a total flavonoids ointment topical application from Pouzolzia zeylanica var. microphylla, on mice skin infections,” Brazilian Journal of Pharmacology, vol. 28, no. 3, pp. 732-737, 2018.

[11] A. R. S. Ibrahim, A. M. Galal, M. S. Ahmed, and G. S. Mossa, “Demethylation and sulfation of 7-methoxylated flavanones by Cunninghamella elegans,” Chemical and Pharmaceutical Bulletin, vol. 51, pp. 203-206, 2003.

[12] J. Gupta and A. Gupta, “Isolation and characterization of flavonoid glycoside from leaves of Abrus precatorius,” International Journal of Chemical Studies, vol. 4, no. 1, pp. 14-17, 2016.

[13] P. S. Kumar, “Bauerenol, a triterpenoid from Indian Suregada angustifolia: Induces reactive oxygen species–mediated P38MAPK activation and apoptosis in human hepatocellular carcinoma (HepG2) cells,” Tumor Biology, vol. 39, no. 4, 2017, doi: 10.1177/1010428317698387.

DOI: https://doi.org/10.34238/tnu-jst.8806

Refbacks

There are currently no refbacks.



Remember me