SURVEYING THE ABILITY OF Bacillus velezensis  OM017175 TO CONTROL THE BACTERIAL LEAF SPOT ON BANANA PEPPER (Capsicum annuum) IN VITRO AND GREEN HOUSE CONDITIONS

Phạm Phú Quí; Đoàn Thị Kiều Tiên; Lê Uyển Thanh; Nguyễn Thị Thu Nga; Hồ Lệ Thi

doi:10.34238/tnu-jst.8870

SURVEYING THE ABILITY OF Bacillus velezensis OM017175 TO CONTROL THE BACTERIAL LEAF SPOT ON BANANA PEPPER (Capsicum annuum) IN VITRO AND GREEN HOUSE CONDITIONS

About this article

Received: 30/09/23 Revised: 29/11/23 Published: 30/11/23

Authors

1. Pham Phu Qui, College of Agriculture - Can Tho University
2. Doan Thi Kieu Tien , College of Agriculture - Can Tho University
3. Le Uyen Thanh, Dong Thap University
4. Nguyen Thi Thu Nga, College of Agriculture - Can Tho University
5. Ho Le Thi, College of Agriculture - Can Tho University

Abstract

The antagonistic ability of Bacillus velezensis OM017175 against Xanthomonas spp. was investigated under in vitro and green-house conditions to find out the effective antagonist population and timing application in controlling bacterial leaf spot on chilli. In vitro, survey results showed that B. velezensis OM017175 treated with inoculum at 10⁹ and 10⁸ CFU/mL has high antagonistic ability and was similarly with inhibition zone ranged from 5.4 to 4.8 mm, respectively. In green-house conditions, two effective timing applications for antagonistic bacteria were determined at 3 days before pathogen inoculation and 3 days after pathogen inoculation. In addition, two treatments treating antagonistic bacteria with populations of 10⁸ or 10⁹ CFU/mL at 3 days after pathogen inoculation still had high disease reduction efficiency, ranging from 40.33% to 40.57%. At 3 days before inoculation, the treatment with 10⁹ CFU/mL was the highest disease reduction efficiency (40.65%). In general, B. velezensis OM017175 has the ability in controlling bacterial leaf spot disease on pepper, with efficiency depending on population and timing application, and has potential to become a biological control agent.

Keywords

Bacillus velezensis; Bacterial leaf spot; Bacterial population; Chilli; Treating time; Xanthomonas sp.

Full Text:

PDF (Tiếng Việt)

References

[1] A. T. P. Mai, Vegetables and vegetable growing. Hanoi Agriculture Publishing House, Hanoi, 1996.

[2] L. Sahitya, D. R. Sri, and M. S. R. Krishna, “Anthracnose, a Prevalent Disease in Capsicum,” Research Journal of Pharmaceutical, Biological and Chemical Sciences, vol. 5, no. 3, 2014, Art. no. 1583.

[3] N. H. Tran and T. L. T. Nguyen, “Research on production of Trichoderma sp. products for controlling the anthracnose disease caused by Colletotrichum spp. on chili (Capsicum frutescens),” CTU Journal of Science, vol. 45, pp. 86-92, 2016.

[4] T. B. Duong, Techniques for growing tomatoes and cucumbers. Phuong Dong Publishing House, 2015.

[5] T. L. Nguyen, “Isolation and selection of antagonistic bacteria from rhizosphere soil against Colletotrichum sp. causing anthracnose on chilli,” CTU Journal of Science, vol. 47, pp. 16-23, 2016.

[6] H. S. Silva, R. S. Romeiro, R. C. Filho, J. L. Pereira, E. S. Mizubuti, and A. Mounteer, “Induction of systemic resistance by Bacillus cereus against tomato foliar diseases under field conditions,” Journal of Phytopathology, vol. 152, pp. 371-375, 2004.

[7] L. S. Murate, A. G. de Oliveira, A. Y. Higashi, A. R. Barazetti, A. S. Simionato, C. S. da Silva, and G. Andrade, “Activity of secondary bacterial metabolites in the control of citrus canker,” Agricultural Sciences, vol. 6, no. 03, 2015, Art. no. 295.

[8] K. V. Pham, The curriculum of general microorganisms, College of Agriculture and applied biology, Can Tho University, p. 159, 2000.

[9] J. W. Kloepper, C.-M. Ryu, and S. A. Zhang, “Induced systemic resistance and promotion of plant growth by Bacillus spp.,” Phytopathology, vol. 94, no. 11, pp. 1259-1266, 2004.

[10] M. R. Roberts and J. E. Taylor, “Exploiting plant induced resistance as a route_ to sustainable crop protection,” In D. B. Collinge (Ed.), Plant pathogen resistance biotechnology, 1st Edition. John Wiley & Sons, Inc., pp. 319-339, 2016.

[11] T. U. Le, “Isolation of Xanthomonas spp. causing leaf spot on rose (Rosa spp.) and pepper (Capsicum spp.) in Dong Thap province and selection of antagonistic bacteria for prevention and control of these diseases,” Doctoral dissertation, College of Agriculture and applied biology, Can Tho University, Can Tho, 2023.

[12] QCVN 01-38: 2010/BNNPTNT, “National technical regulation on Surveillance method of plant pests,” Ha Noi, 2010.

[13] M. J. Jeger and S. L. H. Viljanen-Rollinson, “The use of the area under the disease progress curve (AUDPC) to assess quantitative disease resistance in crop cultivars,” Theoretical and Applied Genetics, vol. 102, no. 1, pp. 32-40, 2001.

[14] W. S. Abbott, “A method of computing the effectiveness of an insecticide,” J. econ. Entomol, vol. 18, no. 2, pp. 265-267, 1925.

[15] M. Mirik, Y. Aysan, and O. Cinar, “Biological control of bacterial spot disease of pepper with Bacillus strains,” Turkish Journal of Agriculture and Forestry, vol. 32, no. 5, pp. 381-390, 2008.

[16] T. P. Huang, D. D. S. Tzeng, A. C. Wong, C. H. Chen, K. M. Lu, Y. H. Lee et al., “DNA Polymorphisms and Biocontrol of Bacillus Antagonistic to Citrus Bacterial Canker with Indication of thef Interference of Phyllosphere Biofilms,” PLoS ONE, vol. 7, no. 7, 2012, Art. no. e42124, doi: 10.1371/journal.pone.0042124.

DOI: https://doi.org/10.34238/tnu-jst.8870

Refbacks

There are currently no refbacks.



Remember me