SOLATION OF BACTERIA FROM SHRIMP POND BOTTOM SLUDGE  WITH THE CAPACITY TO INHIBIT Salmonella enterica subsp enterica serovar Enteritidis ATCC 49223 AND Vibrio spp.

Trương Thị Bích Vân; Nguyễn Thị Loan Anh

doi:10.34238/tnu-jst.8897

SOLATION OF BACTERIA FROM SHRIMP POND BOTTOM SLUDGE WITH THE CAPACITY TO INHIBIT Salmonella enterica subsp enterica serovar Enteritidis ATCC 49223 AND Vibrio spp.

About this article

Received: 04/10/23 Revised: 07/03/24 Published: 12/03/24

Authors

1. Truong Thi Bich Van , Institute of Food and Biotechnology - Can Tho University
2. Nguyen Thi Loan Anh, Institute of Food and Biotechnology - Can Tho University

Abstract

The study isolated 49 bacteriophage strains across nine host strains by double-layer agar–drop method. Overall, all 49 bacteriophage strains are capable of infecting at least one bacterial host. Bacteriophage (ɸ) Pm5 and ɸPm12 are the two phage strains with the widest host spectrum (9/9 and 8/9 bacterial strains respectively), but the small diameter and turbid plaque indicate that the infectiousness of these two phage strains is quite low. ɸVT232 and ɸ5A were discovered to be two new bacteriophage strains that have the ability to specifically detoxify S. Enteritidis and S. Typhimurium. However, ɸ5A is still known as a bacteriophage with many host organisms including Vibrio and E.coli. Phage VT232 was discovered as an antibiotic replacement therapy in the management of due to its stability in the pH range of 5-10 and temperatures of -20ºC to 60ºC. Bacteriophage show promise as a potential agent against Salmonella-induced infections in livestock and aquaculture.

Keywords

Bacteriophage; Bacterial host; Double-layer agar; Salmonella spp.; Vibrio spp.

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DOI: https://doi.org/10.34238/tnu-jst.8897

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