EVALUATION OF THE PROBIOTIC PRODUCTION POTENTIAL  OF BACILLUS SUBTILIS MHN21 STRAIN IN CHICKEN FARMING

Nguyễn Thị Phương Thảo; Nguyễn Thị Ngọc Anh; Vũ Kim Thoa; Tạ Thị Thu Thủy

doi:10.34238/tnu-jst.8965

EVALUATION OF THE PROBIOTIC PRODUCTION POTENTIAL OF BACILLUS SUBTILIS MHN21 STRAIN IN CHICKEN FARMING

About this article

Received: 10/10/23 Revised: 14/11/23 Published: 15/11/23

Authors

1. Nguyen Thi Phuong Thao , Hanoi Open University
2. Nguyen Thi Ngoc Anh, Hanoi Open University
3. Vu Kim Thoa, Hanoi Open University
4. Ta Thi Thu Thuy, Hanoi Open University

Abstract

Strain MHN21 isolated from soil sample at Thai Nguyen chicken farm has high Salmonella resistance properties, and was sequenced gene for identification. The 16S rRNA gene sequence of strain MHN21 is 100% similar to the Bacillus subtilis species, so it was named Bacillus subtilis MHN21, registered on Genbank with code DNW2F69T013. Strain MHN21 is evaluated the probiotic potential invitro conditions to produce products for application in broiler chicken farming. The results showed that the research strain has good characteristics such as: producing two extracellular enzymes (amylase, protease) have high activity with a diameter of substrate resolution from 18 - 21mm; resistance to gastrointestinal pathogenic bacteria Staphylococcus aureus, Escherichia coli, Salmonella with zone of inhibition diameter 12 - 22mm; sensitive to 3 types of antibiotics Ampicillin, Chloramphenicol, Tetracycline; can withstand environment pH 2.0 and bile salts 2%, after 24 hours, survival rate reaches over 80%; good adhesion to chicken intestinal epithelium. Bacillus subtilis MHN21 strain has the potential for research into creating probiotic products for chicken farming.

Keywords

Bacillus; Probiotic; Microorganism; Chicken; Production

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DOI: https://doi.org/10.34238/tnu-jst.8965

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