EFFECTS OF STORAGE MEDIUM AND STORAGE TIME ON THE GOAT SPERMATOZOA QUALITY

Nguyễn Lâm Khánh Duy; Bùi Thanh Liêm; Ngô Phạm Nhật Vy; Trần Thị Thanh Khương

doi:10.34238/tnu-jst.9186

EFFECTS OF STORAGE MEDIUM AND STORAGE TIME ON THE GOAT SPERMATOZOA QUALITY

About this article

Received: 11/11/23 Revised: 05/03/24 Published: 11/03/24

Authors

1. Nguyen Lam Khanh Duy, Institute of Food and Biotechnology - Can Tho University
2. Bui Thanh Liem, Institute of Food and Biotechnology - Can Tho University
3. Ngo Pham Nhat Vy, Institute of Food and Biotechnology - Can Tho University
4. Tran Thi Thanh Khuong , Institute of Food and Biotechnology - Can Tho University

Abstract

The storage medium for liquid semen and storage time play an important role in maintaining and stabilizing sperm quality during storage. The objective of the study was to determine the carbon source in the appropriate storage environment and optimal storage time to preserve the best sperm quality. The study evaluated the role of three different carbon sources in the storage medium: glucose, fructose and sucrose. This study included 6 treatments corresponding to 6 types of storage media, each solution was repeated 8 times. After collection, semen samples were diluted with storage medium at a ratio of 1:10, then the samples were stored at 15°C. The quality assessment according to specific time points were 0 hours, 6 hours, 12 hours, 24 hours, 48 hours and 72 hours. Research results showed that sperm quality decreases with storage time. The results of microscopic evaluation showed that, after 72 hours of storage, the best quality of goat sperm was recorded in Tris Citrate Sucrose-2 medium. Specifically, the overall motility rate was 71.61%, progressive motility rate was 64.91%, sperm viability rate was 74.50% and the membrane integrity was 57.59%. The results showed that sucrose improved sperm quality better than glucose and fructose, Tris Citrate Sucrose-2 medium helped keep the best sperm quality and the appropriate storage time was 72 hours.

Keywords

Carbon source; Goat; Storage medium; Semen; Sperm

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DOI: https://doi.org/10.34238/tnu-jst.9186

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