EFFECT OF EXTRACTION SOLVENT TO TOTAL POLYPHENOL CONTENT AND ANTIOXIDANT ACTIVITY OF GUAVA LEAF EXTRACT

Nguyễn Thị Như Ý; Nguyễn Như Muội; Huỳnh Nguyễn Thuý Quỳnh; Đoàn Thị Kiều Tiên; Đỗ Thị Tuyết Nhung; Nguyễn Cường Quốc; Trần Quang Đệ; Lê Thanh Phước; Phạm Vũ Nhật

doi:10.34238/tnu-jst.9190

EFFECT OF EXTRACTION SOLVENT TO TOTAL POLYPHENOL CONTENT AND ANTIOXIDANT ACTIVITY OF GUAVA LEAF EXTRACT

About this article

Received: 12/11/23 Revised: 29/01/24 Published: 30/01/24

Authors

1. Nguyen Thi Nhu y , 1) Can Tho University of Technology, 2) Can Tho University
2. Nguyen Nhu Muoi, Can Tho University of Technology
3. Huynh Nguyen Thuy Quynh, Can Tho University of Technology
4. Doan Thi Kieu Tien, Can Tho University of Technology
5. Do Thi Tuyet Nhung, Can Tho University of Technology
6. Nguyen Cuong Quoc, Can Tho University
7. Tran Quang De, Can Tho University
8. Le Thanh Phuoc, Can Tho University
9. Pham Vu Nhat, Can Tho University

Abstract

This study investigated the effect of extraction solvent on total polyphenol content and antioxidant activity of Queen guava leaf extract. Theleaves were collected in Bac Lieu province, washed, dried and ground to obtain powder as raw material. Two types of solvents, namelyethanol and methanol, were used to extract by maceration method. The obtained products were determined extraction efficiency and total polyphenol content. Theresults showed that methanol extract acquired higher extraction efficiency and polyphenol content compared to ethanol extract. Methanol extract was selected for the investigation of fraction extracts in three solvents with different polarities: n-hexane, dichloromethane (DC) and ethyl acetate (EA). The antioxidant activity of methanol, n-hexane, DC and EAextracts was identified by DPPH and ABTS assays. The antioxidant activity of total methanol and EA extracts were the best with EC₅₀ values of 15.39 and 12.49 µg/mL,respectively. Theseresults facilitate selectingsuitable solvent in developing natural antioxidant products from Queen guava leaf.

Keywords

Queen guava; Guava leaf extract; Extraction solvent; Polyphenol content; Antioxidant activity

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DOI: https://doi.org/10.34238/tnu-jst.9190

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