CHARACTERISTICS OF THE MORPHOLOGY, ANATOMY  AND matK GENE REGION OF Cassia fistula (L.) PLANTS

Vũ Thị Thu Thuỷ; Xyphanboun Viengdavanh; Trần Thị Hồng; Nguyễn Thị Thu Ngà; Lò Thị Mai Thu; Chu Hoàng Mậu

doi:10.34238/tnu-jst.9291

CHARACTERISTICS OF THE MORPHOLOGY, ANATOMY AND matK GENE REGION OF Cassia fistula (L.) PLANTS

About this article

Received: 27/11/23 Revised: 05/03/24 Published: 11/03/24

Authors

1. Vu Thi Thu Thuy, TNU - University of Education
2. Xyphanboun Viengdavanh, TNU - University of Education
3. Tran Thi Hong, TNU - University of Education
4. Nguyen Thi Thu Nga, TNU - University of Education
5. Lo Thi Mai Thu, Taybac University
6. Chu Hoang Mau , TNU - University of Education

Abstract

This study aims to identify Cassia plant samples collected in Thai Nguyen based on morphological and anatomical analysis methods and the support of chloroplast DNA barcoding. The Cassia fistula sample collected in Thai Nguyen has a woody trunk to 15m tall and many branches; leaves are compound, 20-40 cm long, broadly ovate or ovate-oblong with a pointed tip. Racemes axillary, 20-60 cm, many flowered. Pedicels 3-5 cm, slender. Sepals narrowly ovate, reflexed at anthesis. Petals are golden yellow, broadly ovate, subequal, and shortly clawed. Stamens 10, three long with curved filaments 3-4 cm, anthers about 5 mm long, exceeding petals, four short with straight filaments 6-10 mm, reduced stamens with minute anthers. Ovary stalked, strigulose, stigma small. The fruit is bean-shaped, blackish brown, dark brown when ripe, 30-60 cm long, 2-2.5 cm in diameter. Each fruit has many seeds, separated by septa. Seeds are oval, flat, and shiny brown. Microscopic specimens of the leaves and stems of the Cassia reveal the following parts: epidermis, thick tissue, soft tissue, hard tissue, phloem, xylem, and gut. The matK gene region was amplified by PCR from young leaves of the Cassia sample with a size of 816 nucleotides. Phylogenetic analysis based on the sequence of the matK gene region shows that the Cassia sample collected in Thai Nguyen is in the same group as the C. fistula species with code MG737426.1 on GenBank with the bootstrap coefficient of 98%. The morphological, anatomical and sequence data of the matK gene region are the basis for determining that the Cassia sample collected in Thai Nguyen belongs to the C. fistula species.

Keywords

Cassia fistula; matK gene region; Plant microsurgery; Plant morphology; Species identification

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DOI: https://doi.org/10.34238/tnu-jst.9291

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