EVALUATION OF INDIGOTIN CONTENT IN INDIGO POWDER COLLECTED FROM TWO PLANT SPECIES DISTRIBUTED IN LAM DONG PROVINCE

Nguyễn Văn Ngọc; Võ Văn Nghĩa; Lê Trúc Linh; Hoàng Thị Bình

doi:10.34238/tnu-jst.9547

EVALUATION OF INDIGOTIN CONTENT IN INDIGO POWDER COLLECTED FROM TWO PLANT SPECIES DISTRIBUTED IN LAM DONG PROVINCE

About this article

Received: 05/01/24 Revised: 10/06/24 Published: 11/06/24

Authors

1. Nguyen Van Ngoc , Dalat University
2. Vo Van Nghia, Dalat University; Tay Nguyen research center of medicinal materials - National Institute of medicinal materials
3. Le Truc Linh, Dalat University
4. Hoang Thi Binh, Dalat University

Abstract

Indigofera cassioides Rottl. ex Bl. and Indigofera suffruticosa Mill., which are widely distributed in Lam Dong province, serve as a natural source of indigo color. Indigo powder, known for its inherent indigo color, has been used by humans for dyeing and medicinal purposes for a long time. However, no global research has investigated the extraction efficiency of indigo powder and the indigotin content from these two indigo species. This study aimed to evaluate the extraction efficiency of indigo powder and measure the indigotin content in both the powder and fresh leaves of these species in Lam Dong. The indigo powder was obtained using the maceration extraction method, and the indigotin content was determined through spectrophotometry using a JASCO V-730 spectrophotometer (UV-VIS). The results showed that the efficiency of extracting and collecting indigo powder from these two species was 4.61 ± 0.30% for I. cassioides and 4.23 ± 0.92% for I. suffruticosa. The indigotin content in I. cassioides indigo powder was found to be 2.83 ± 0.467%, while in I. suffruticosa indigo powder, it was 8.80 ± 2.12%. This corresponds to an indigotin content in fresh leaves of I. cassioides and I. suffruticosa samples of 0.13 ± 0.02% and 0.37 ± 0.09%, respectively.

Keywords

Indigotin; Indigo powder; Indigofera cassioides; Indigofera suffruticosa; Lam Dong

Full Text:

PDF (Tiếng Việt)

References

[1] P. Guinot, A. Rogé, A. Gargadennec, M. Garcia, D. Dupont, E. Lecoeur, and C. Andary, “Dyeing plants screening: an approach to combine past heritage and present development,” Coloration Technology, vol. 122, no. 2, pp. 93-101, 2006.

[2] S. Ali, T. Hussain, and R. Nawaz, “Optimization of alkaline extraction of natural dye from Henna leaves and its dyeing on cotton by exhaust method,” Journal of cleaner production, vol. 17, no.1, pp. 61-66, 2009.

[3] T. Maugard, E. Enaud, P. Choisy, and M. D. Legoy, “Identification of an indigo precursor from leaves of Isatis tinctoria (Woad),” Phytochemistry, vol. 58, no. 6, pp. 897-904, 2001.

[4] A. Verhecken, “Experiments with the dyes from European purple-producing molluscs,” Dyes in History and Archaeology, vol. 12, pp. 32-35, 1994.

[5] J. C. Splitstoser, T. D. Dillehay, J. Wouters, and A. Claro, “Early pre-Hispanic use of indigo blue in Peru,” Science advances, vol. 2, no. 9, 2016, Art. no. e1501623.

[6] Y. K. Lin, C. J. Chang, Y. C. Chang, W. R. Wong, S. C. Chang, and J. H. S. Pang, “Clinical evaluation of patients with persistent psoriasis in a randomized, blind, observational, vehicle-controlled trial using natural indigo,” Archives of dermatology, vol. 144, no. 11, pp. 1457-1464, 2008.

[7] F. C. Lopes, T. R. Calvo, L. L. Colombo, W. Vilegas, and I. Z. Carlos, “Immunostimulatory and cytotoxic activities of Indigofera suffruticosa (Fabaceae),” Natural Product Research, vol. 25, no. 19, pp. 1796-1806, 2011.

[8] M. N. MacDonald, “Indigo planting in India,” Pearsons Magazine, vol. 10, pp. 387-392, 1900.

[9] L. G. Angelini, E. Campeol, S. Tozzi, S. K. G. Gilbert, D. T. Cooke, and P. John, “A New HPLC‐ELSD Method To Quantify Indican in Polygonum tinctorium L. and To Evaluate β‐Glucosidase Hydrolysis of Indican for Indigo Production,” Biotechnology progress, vol. 19, no. 6, pp. 1792-1797, 2003.

[10] C. T. Muzzazinah and N. S. Ariyanti, “Correlation of morphological characteristics with the presence of indicant in Indigofera sp. Dyestuff,” Sains Malaysiana, vol. 45, no. 6, pp. 883-890, 2016.

[11] Y. Shin, D. I. Yoo, and K. Kim, “Process balance of natural indigo production based on traditional Niram method,” Journal of The Korean Society of Dyers and Finishers, vol. 24, no. 4, pp. 253-259, 2012.

[12] N. Comlekcioglu, L. Efe, and S. Karaman, “Extraction of indigo from some Isatis species and dyeing standardization using low-technology methods,” Brazilian Archives of Biology and Technology, vol. 58, pp. 96-102, 2015.

[13] H. H. Pham, An illustrated flora of Vietnam, vol. 1. Young Publishing House, Ho Chi Minh City, 2003, pp. 911-912.

[14] T. L. Do, Vietnamese medicinal herbs. Medical Publishing House, 2004, pp.114-116.

[15] V. D. Nguyen and V. T. Nguyen, Chemical research methods of medicinal plants. Medical Publishing House, 1985.

[16] W. S. Laitonjam and S. D. Wangkheirakpam, “Comparative study of the major components of the indigo dye obtained from Strobilanthes flaccidifolius Nees. and Indigofera tinctoria Linn,” International Journal of Plant Physiology and Biochemistry, vol. 3, no. 7, pp. 108-116, 2011.

DOI: https://doi.org/10.34238/tnu-jst.9547

Refbacks

There are currently no refbacks.



Remember me