A REPORT ON THE CREATION OF THE COLLECTION OF HOST OPTIMIZED GENES ENCODING FOR FLAVIVIRUS GLOBAL CONSENSUS ENVELOPE (E) AND NON-STRUCTURAL PROTEIN 1 (NS1)

Cù Lê Nguyên; Đặng Tuấn Đạt; Nguyễn Ngọc Lương

doi:10.34238/tnu-jst.9643

A REPORT ON THE CREATION OF THE COLLECTION OF HOST OPTIMIZED GENES ENCODING FOR FLAVIVIRUS GLOBAL CONSENSUS ENVELOPE (E) AND NON-STRUCTURAL PROTEIN 1 (NS1)

About this article

Received: 26/01/24 Revised: 14/05/24 Published: 17/05/24

Authors

1. Cu Le Nguyen, Buon Ma Thuot Medical University
2. Dang Tuan Dat, Buon Ma Thuot Medical University
3. Nguyen Ngoc Luong , Hue University - College of Sciences

Abstract

Vietnam is a critical region for infectious diseases, notably those caused by Flaviviruses, which are responsible for severe outbreaks of dengue fever, Japanese encephalitis, and Zika virus-induced microcephaly. Seroepidemiological studies in Vietnam are hindered by substantial challenges, leading to less effective public health strategies and preventative actions. A primary obstacle is the scarcity of affordable, high-quality antigens needed for extensive serological assessments. Additionally, the diversity of the virus strains in circulation complicates the development of universally applicable diagnostics and preventative tools. To overcome these issues, our study aimed to generate a suite of codon-optimized genes that encode the global consensus E glycoprotein and NS1 non-structural protein from three viruses endemic to Vietnam: Dengue, Zika, and Japanese Encephalitis viruses. This paper details the methodology for constructing the global consensus proteins E and NS1, optimizing the genes for various expression hosts, and presents initial expression outcomes in various Escherichia coli strains.

Keywords

Flavivirus; Protein E; Protein NS1; Global consensus antigens; Seroepidemiological surveys

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DOI: https://doi.org/10.34238/tnu-jst.9643

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