PURPLE NONSULFUR BACTERIUM STRAIN RHODOBACTER SPHAEROIDES ISOLATED FROM NAM DINH SHRIMP POND CAPACITY PRODUCING ENZYMES AND ANTAGONISM TO VIBRIO PARAHAEMOLYTICUS

Nguyễn Thị Thu Huyền; Phạm Thanh Trang; Đinh Thị Thu Hằng; Trần Thu Hà; Hoàng Thị Yến

doi:10.34238/tnu-jst.10215

PURPLE NONSULFUR BACTERIUM STRAIN RHODOBACTER SPHAEROIDES ISOLATED FROM NAM DINH SHRIMP POND CAPACITY PRODUCING ENZYMES AND ANTAGONISM TO VIBRIO PARAHAEMOLYTICUS

About this article

Received: 25/04/24 Revised: 31/05/24 Published: 06/06/24

Authors

1. Nguyen Thi Thu Huyen , TNU – University of Sciences
2. Pham Thanh Trang, Institute of Biotechnology - Vietnam Academy of Science and Technology
3. Dinh Thi Thu Hang, Graduate University of Sciences and Technology - Vietnam Academy of Science and Technology
4. Tran Thu Ha, Institute of Biotechnology - Vietnam Academy of Science and Technology; Graduate University of Sciences and Technology - Vietnam Academy of Science and Technology
5. Hoang Thi Yen, Institute of Biotechnology - Vietnam Academy of Science and Technology

Abstract

Aquaculture has been faced with huge losses in productivity caused by diseases and water pollution. Therefore, the selection of bacteria strains that have both the ability to against pathogenic bacteria and producing enzymes to use as probiotics for the decomposition of organic matter, and reduce water pollution has been studied. Purple nonsulfur bacterium strain ND42 can inhibit Vibrio parahaemolyticus that causes AHPND with an inhibition zone diameter of 14.66±0.31 mm and capable of producing 2 enzymes gelatinase and proteinase with clear zones diameters of 3.38±0.64 mm and 5.23±1.40 mm, respectively. Using the 16S rRNA gene sequencing, strain ND42 may belong to the species Rhodobacter sphaeroides and was named Rhodobacter sphaeroides ND42.

Keywords

Purple nonsulfur bacteria; Antivibrio compounds; Bacterial enzymes; Rhodobacter sphaeroides; V. parahaemolyticus

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References

[1] T. Thanh, “Total seafood production in 2023 is estimated to reach over 9,312 thousand," Directorate of fisheries, January 17, 2024. [Online]. Available: https://tongcucthuysan.gov.vn/vi-vn. [Accessed Apr. 16, 2024].

[2] L. Tran et al., “Determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp,” Dis. Aquat. Organ., vol. 105, no. 1, pp. 45-55, Jul. 2013, doi: 10.3354/dao02621.

[3] S. Panwichian, D. Kantachote, B. Wittayaweerasak, and M. Mallavarapu, “Factors affecting immobilization of heavy metals by purple nonsulfur bacteria isolated from contaminated shrimp ponds,” World J. Microbiol. Biotechnol., vol. 26, no. 12, pp. 2199-2210, Dec. 2010, doi: 10.1007/s11274-010-0405-8.

[4] F. Egger, T. Hülsen, S. Tait, and D. J. Batstone, “Autotrophic sulfide removal by mixed culture purple phototrophic bacteria,” Water Res., vol. 182, Sep. 2020, Art. no. 115896, doi: 10.1016/j.watres.2020.115896.

[5] N. Rashid, M. N. A. Abdelnabi, A. S. Vincent, and H. R. Mackey, “Simultaneous treatment of fruit juice industry wastewater and single-cell protein synthesis using purple non-sulfur bacteria,” Biomass Convers. Biorefinery, vol. 13, no. 18, pp. 16321-16332, Dec. 2023, doi: 10.1007/s13399-022-03669-6.

[6] Z. W. LaTurner, G. N. Bennett, K.-Y. San, and L. B. Stadler, “Single cell protein production from food waste using purple non-sulfur bacteria shows economically viable protein products have higher environmental impacts,” J. Clean. Prod., vol. 276, Dec. 2020, Art. no. 123114, doi: 10.1016/j.jclepro.2020.123114.

[7] O. Z. Wada, A. S. Vincent, and H. R. Mackey, “Single-cell protein production from purple non-sulphur bacteria-based wastewater treatment,” Rev. Environ. Sci. Biotechnol., vol. 21, no. 4, pp. 931-956, Dec. 2022, doi: 10.1007/s11157-022-09635-y.

[8] S. Torpee, D. Kantachote, A. Sukhoom, and M. Tantirungkij, “Culture optimization to enhance carotenoid production of a selected purple nonsulfur bacterium and its activity against acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus,” Biotechnol. Appl. Biochem., vol. 69, no. 6, pp. 2422-2436, 2022, doi: 10.1002/bab.2292.

[9] A. Koga, M. Goto, S. Hayashi, S. Yamamoto, and H. Miyasaka, “Probiotic Effects of a Marine Purple Non-Sulfur Bacterium, Rhodovulum sulfidophilum KKMI01, on Kuruma Shrimp (Marsupenaeus japonicus),” Microorganisms, vol. 10, no. 2, Feb. 2022, Art. no. 2, doi: 10.3390/microorganisms10020244.

[10] H. Miyasaka, A. koga, and T. Maki, “Recent progress in the use of purple non-sulfur bacteria as probiotics in aquaculture,” World J. Microbiol. Biotechnol., vol. 39, no. 6, p. 145, Apr. 2023, doi: 10.1007/s11274-023-03592-6.

[11] A. Kumar, “Studies on purple non sulfur bacteria from shrimp ponds and their potential applications,” INFLIBNET, 2012. [Online]. Available: https://shodhganga.inflibnet.ac.in:8443/jspui/handle/10603/9637. [Accessed Apr. 25, 2024].

[12] A. Alloul, M. Wille, P. Lucenti, P. Bossier, G. Van Stappen, and S. E. Vlaeminck, “Purple bacteria as added-value protein ingredient in shrimp feed: Penaeus vannamei growth performance, and tolerance against Vibrio and ammonia stress,” Aquaculture, vol. 530, Jan. 2021, Art. no. 735788, doi: 10.1016/j.aquaculture.2020.735788.

[13] S. Chumpol, D. Kantachote, T. Nitoda, and H. Kanzaki, “The roles of probiotic purple nonsulfur bacteria to control water quality and prevent acute hepatopancreatic necrosis disease (AHPND) for enhancement growth with higher survival in white shrimp (Litopenaeus vannamei) during cultivation,” Aquaculture, vol. 473, pp. 327-336, Apr. 2017, doi: 10.1016/j.aquaculture.2017.02.033.

[14] U. T. T. Do, Y. T. Hoang, L. T. Do, and N. V. Tran, "Study on photosynthetic bacteria as living feed in quaacuture breeding," Journal of Biotechnology, vol. 12, pp. 41-45, 2009.

[15] L. T. Do, "Study on application of purple photosynthetic bacteria for removal of sulfide from wastewater," PhD Thesis, Vietnam National University, 2016.

[16] Y. T. Hoang, “Study on purple nonsulfur bacteria isolated in Vietnam for use in aquaculture,” PhD Thesis, Vietnam National University, Hanoi, 2010.

DOI: https://doi.org/10.34238/tnu-jst.10215

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