RESEARCH ON CHARACTERISTICS OF THE 18S rRNA PARTIAL GENE, THE rbcL GENE AND PHYLOGENETIC RELATIONSHIP OF Paris vietnamensis COLLECTED IN YEN BAI

Abstract

The Paris vietnamensis is one of the important medicinal herbs that was used in traditional medicine. In this study, the partial 18S rRNA gene and rbcL gene of the P. vietnamensis collected in Yen Bai were isolated, sequenced, and characterized. Moreover, the study also evaluated the potential application of 18S rRNA and rbcL to classify, identify, and phylogeny of species. The results showed that the length of 18S rRNA and rbcL were 973 bp and 563 bp, respectively; GC content of these two gene regions was 50.98 (%) and 44.23 (%). Analysis by BLAST program with the published 18S rRNA and rbcL sequences of the Paris genus had shown that the 18S rRNA gene region of this study had high similarity from 99.08 to 99.28% and contained two conserved sequences that were from 84 to 604 and from 606 to 871. The rbcL gene had high similarity (99.82%) to the published rbcL sequences of the Paris genus. The 18S rRNA gene region may be a potential DNA barcode candidate to support classification and identification at the species level, while the rbcL may support classification and identification at the genus level.